Gene Electroporator GP-3000
Ƙayyadaddun bayanai
| Samfura | Saukewa: GP-3000 |
| Fom ɗin bugun jini | Lalacewar Maɗaukaki da Wave Square |
| Babban ƙarfin lantarki | 401-3000V |
| Ƙarfin wutar lantarki | 50-400V |
| Babban ƙarfin wutan lantarki | 10-60μF a cikin matakan 1μF (10μF, 25μF, 35μF, 50μF, shawarar 60μF) |
| Ƙananan ƙarfin wutar lantarki | 25-1575μF a cikin matakan 1μF (matakan 25μF sun ba da shawarar) |
| Parallel resistor | 100Ω-1650Ω a cikin matakai 1Ω (shawarar 50Ω) |
| Tushen wutan lantarki | 100-240VAC50/60HZ |
| Tsarin aiki | Mai sarrafa kwamfuta |
| Tsawon lokaci | tare da RC lokaci akai-akai, daidaitacce |
| Cikakken nauyi | 4.5kg |
| Girman Kunshin | 58 x 36 x 25 cm |
Bayani
Electroporation ta salula wata hanya ce mai mahimmanci don gabatar da macromolecules na waje irin su DNA, RNA, siRNA, sunadarai, da ƙananan ƙwayoyin cuta zuwa cikin sel membranes.
A ƙarƙashin rinjayar filin lantarki mai ƙarfi na ɗan lokaci, membrane membrane a cikin bayani yana samun wani rauni. Abubuwan da aka caje suna shiga cikin tantanin halitta ta hanyar kama da electrophoresis. Saboda tsananin juriya na phospholipid bilayer na tantanin halitta, ƙarfin wutar lantarki na bipolar da ke haifar da filin lantarki na waje yana ɗaukar membrane tantanin halitta, kuma ƙarfin lantarki da aka rarraba a cikin cytoplasm ana iya yin watsi da shi, kusan babu halin yanzu a cikin cytoplasm. don haka kuma ƙayyade ƙananan ƙwayar cuta a cikin kewayon al'ada na tsarin electrophoresis.
Aikace-aikace
Ana iya amfani dashi don electroporation don canja wurin DNA zuwa ƙwararrun sel, tsirrai da ƙwayoyin dabba, da ƙwayoyin yisti. Kamar electroporation na kwayoyin cuta, yisti, da sauran microorganisms, transfection na mammalian Kwayoyin, da kuma canja wurin da shuka kyallen takarda da protoplasts, cell hybridization da gene fusion gabatarwar, gabatarwar alama genes ga labeling da nuni dalilai, gabatarwar kwayoyi, sunadarai, antibodies. da sauran kwayoyin halitta don nazarin tsarin tantanin halitta da aiki.
Siffar
• Babban inganci: gajeriyar lokacin jujjuyawa, babban juzu'i, babban maimaitawa;
• Ma'ajiyar hankali: na iya adana sigogin gwaji, dacewa ga masu amfani suyi aiki;
Madaidaicin iko: Mai sarrafa bugun jini mai sarrafa microprocessor;Ø
• Kyawawan bayyanar: haɗaɗɗen ƙirar injin gabaɗaya, nunin fahimta, aiki mai sauƙi.
FAQ
Tambaya: Menene Gene Electroporator?
A: Gene Electroporator kayan aiki ne da ake amfani da shi don gabatar da kayan halitta na waje, irin su DNA, RNA, da sunadarai, cikin sel ta hanyar tsarin lantarki.
Tambaya: Wadanne nau'ikan sel ne za a iya yi niyya tare da Gene Electroporator?
A: Za a iya amfani da Mai Electroporator na Halitta don shigar da kwayoyin halitta cikin nau'ikan tantanin halitta iri-iri da suka hada da kwayoyin cuta, yisti, kwayoyin shuka, kwayoyin dabbobi masu shayarwa, da sauran kwayoyin halitta.
Tambaya: Menene manyan aikace-aikace na Gene Electroporator?
A:
• Electroporation na ƙwayoyin cuta, yisti, da sauran ƙananan ƙwayoyin cuta: Don canjin kwayoyin halitta da nazarin aikin kwayoyin halitta.
• Canja wurin sel masu shayarwa, kyallen tsire-tsire, da protoplasts: Don nazarin maganganun kwayoyin halitta, kwayoyin halitta masu aiki, da injiniyan kwayoyin halitta.
• Gabatarwar haɗaɗɗun ƙwayoyin halitta da haɓakar ƙwayoyin halitta: Don ƙirƙirar sel masu haɗaɗɗun ƙwayoyin cuta da gabatar da ƙwayoyin fusion.
Gabatarwa na kwayoyin halitta masu alama: Don yin lakabi da bin diddigin maganganun kwayoyin halitta a cikin sel.
Gabatarwa na kwayoyi, sunadarai, da ƙwayoyin rigakafi: Don bincika tsarin kwayar halitta da aiki, bayarwa na miyagun ƙwayoyi, da nazarin hulɗar furotin.
Tambaya: Ta yaya Gene Electroporator ke aiki?
A: Gene Electroporator yana amfani da gajeriyar bugun jini mai ƙarfi mai ƙarfi don ƙirƙirar pores na wucin gadi a cikin tantanin halitta, yana barin ƙananan ƙwayoyin cuta su shiga cikin tantanin halitta. Lambun tantanin halitta yana sake dawowa bayan bugun bugun wutar lantarki, yana kama abubuwan da aka gabatar a cikin tantanin halitta.
Tambaya: Menene fa'idodin amfani da Gene Electroporator?
A: Babban maimaitawa da inganci, sauƙi na aiki: Sauƙaƙan tsari da sauri, sarrafawa mai ƙididdigewa, babu genotoxicity: Ƙananan yuwuwar lalacewa ga kwayoyin halitta ta tantanin halitta.
Tambaya: Za a iya amfani da Gene Electroporator don kowane nau'in gwaji?
A: Yayin da Gene Electroporator ke da yawa, ingancinsa na iya bambanta dangane da nau'in tantanin halitta da kuma kwayoyin halittar da ake gabatarwa. Yana da mahimmanci don haɓaka yanayi don kowane takamaiman gwaji.
Tambaya: Wane kulawa ta musamman ake buƙata bayan gabatarwa?
A: Kulawar bayan gabatarwa na iya haɗawa da shigar da sel a cikin matsakaicin farfadowa don taimaka musu su gyara da ci gaba da ayyuka na yau da kullun. Takamaiman na iya bambanta dangane da nau'in tantanin halitta da gwajin.
Tambaya: Shin akwai wata damuwa ta aminci game da amfani da Gene Electroporator?
A: Ya kamata a bi daidaitattun ayyukan aminci na dakin gwaje-gwaje. Gene Electroporator yana amfani da babban ƙarfin lantarki, don haka dole ne a bi tsarin kulawa da aminci don hana haɗarin lantarki.
