Samfurin Shiri daAna lodawa
Saboda yin amfani da tsarin buffer mai ci gaba ba tare da stacking gel ba a mafi yawan lokuta, samfurori ya kamata su sami nauyin da ya dace da ƙananan ƙarar. Yi amfani da apipettedon ƙara samfurin sannu a hankali, tare da 5-10 μg a kowace rijiya, don kauce wa raguwa mai mahimmanci a ƙuduri. Yausheloading samfurin, dole ne a kashe wutar lantarki. Samfurin ya kamata ya ƙunshi rini mai nuna alama (0.025% bromophenol blue ko orange) da sucrose (10-15%) ko glycerol (5-10%) don haɓaka yawansa, mai da hankali samfurin, da hana yaduwa. Koyaya, wani lokacin sucrose ko glycerol na iya haifar da nau'ikan nau'ikan U-dimbin yawa a cikin sakamakon electrophoresis, wanda za'a iya kauce masa ta amfani da 2.5% Ficoll (polyvinylpyrrolidone).
Electrophoresis
Wutar lantarki don electrophoresis shine 5-15 V / cm, gabaɗaya kusan 10 V / cm. Don rabuwa da manyan kwayoyin halitta, ƙarfin lantarki ya kamata ya zama ƙasa, yawanci bai wuce 5 V / cm ba.
Tabo
Fluorescent dye ethidium bromide (EB) ana yawan amfani dashi don tabo don lura da makada na DNA a cikin gel agarose. EB na iya saka tsakanin tushe nau'i-nau'i na kwayoyin DNA, haifar da EB don ɗaure da DNA. Ana ɗaukar hasken ultraviolet na 260nm ta DNA zuwa EB, kuma EB ɗin da aka ɗaure yana fitar da haske a 590 nm a cikin yankin ja-orange na bakan haske na bayyane. Sanya gel a cikin bayani na 1 mmol/L MgSO4 na awa 1 na iya rage hasken baya da ya haifar ta hanyar EB mara iyaka, yana sauƙaƙe gano ƙananan adadin DNA.
Rini na EB yana da fa'idodi da yawa: yana da sauƙin amfani, baya karya acid nucleic, yana da hankali sosai, kuma yana iya lalata DNA da RNA duka. Ana iya ƙara EB zuwa samfurin kuma ana bin sa ido ta amfani da sha UV a kowane lokaci. Bayan tabo, za a iya cire EB ta hanyar cirewa tare da n-butanol.
Koyaya, rini na EB shine mutagen mai ƙarfi, kuma matakan tsaro, kamar saka safofin hannu na polyethylene, yakamata a ɗauka yayin kulawa. Acridine orange shima rini ne da aka saba amfani dashi domin yana iya bambance tsakanin acid nucleic acid mai dunƙule guda da igiya biyu (DNA, RNA). Yana nuna haske mai haske (530 nm) don acid nucleic masu ɗauri biyu da ja (640 nm) don acid nucleic masu ɗaci ɗaya. Bugu da ƙari, za a iya amfani da wasu rinannun irin su methylene blue, methylene green, da quinoline B don tabo.
Beijing Liuyi Biotechnology Co. Ltd (Liuyi Biotechnology) ya ƙware a masana'antar kayan aikin electrophoresis fiye da shekaru 50 tare da ƙungiyar ƙwararrun ƙwararrunmu da cibiyar R&D. Muna da amintaccen kuma cikakken layin samarwa daga ƙira zuwa dubawa, da ɗakunan ajiya, gami da tallafin talla. Babban samfuranmu sune Electrophoresis Cell (tanki / ɗakin), Samar da wutar lantarki na Electrophoresis, Mai watsawa na LED mai haske, Mai watsawa UV, Gel Image & Analysis System da dai sauransu.
Yanzu muna neman abokan tarayya, duka OEM electrophoresis tank da masu rarraba suna maraba.
Idan kuna da wani shirin siyan samfuranmu, da fatan a yi shakka a tuntuɓe mu. Kuna iya aiko mana da sako ta imel[email protected]ko[email protected], ko kuma a kira mu a +86 15810650221 ko ƙara Whatsapp +86 15810650221, ko Wechat: 15810650221.
Da fatan za a bincika lambar QR don ƙara akan Whatsapp ko WeChat.
Lokacin aikawa: Dec-20-2023