Menene Cellulose Acetate Membrane Electrophoresis?
Cellulose Acetate Membrane Electrophoresis wani nau'i ne na fasaha na electrophoresis wanda ke amfani da membrane acetate cellulose a matsayin mai goyan bayan kafofin watsa labaru don gwaje-gwaje.
Cellulose acetate wani nau'i ne na acetate na cellulose wanda yake acetylated daga cellulose's hydroxyl. Lokacin da yake narkewa a cikin maganin kwayoyin halitta kamar acetone, ana iya shafa shi zuwa fim mai kama da kyau tare da ƙananan pores. Kaurin fim ɗin yana kusa da 0.1-0.15mm wanda ya dace da gwaji.
Amfanin cellulose acetate membrane
Idan aka kwatanta da takarda, yana da fa'idodi masu zuwa:
1.Kyakkyawan sakamako na rabuwa. Don samfuran furotin, membrane acetate cellulose yana ɗaukar kaɗan daga cikinsu, bayan rini, zai iya canza launi gaba ɗaya daga launi na baya. Makadan rini a bayyane suke, don haka yana inganta daidaito don tantance ƙididdigewa.
2.Fast da ajiye lokaci. Acetate membrane cellulose acetate ba shi da hydyophilic fiye da takarda, don haka aikin electro-osmosis bai kai takarda mai walƙiya ba, kuma wutar lantarki ana gudanar da shi ne ta hanyar samfurori wanda zai iya taƙaita lokacin rabuwa da ƙasa da lokacin electrophoresis. Yawanci, lokacin cellulose acetate membrane electrophoresis shine kimanin minti 45-60. Dukkanin tsarin electrophoresis ciki har da rini da canza launi yana buƙatar mintuna 90 kawai.
3.High hankali da ƙananan samfurori da za a yi amfani da su. Yana bukatar kawai 2 μL serum a cikin serum protein electrophoresis.
4.Wide aikace-aikace. Wasu sunadaran ba su da sauƙin rabuwa akan electrophoresis na takarda, kamar Alpha-fetoprotein, bacteriolytic enzyme, insulin, da histone da sauransu. Amma ya fi kyau a yi amfani da cellulose acetate membrane electrophoresis don raba su.
5. Mai sauƙin kiyayewa da ƙididdigewa. Bayan dyeing da cellulose acetate membrane electrophoresis samfurori, nutsad da samfurori a cikin gauraye bayani na glacial acetic acid da ethanol yi wani m busassun farantin da za a ajiye na dogon lokaci.
Saboda cellulose acetate membrane electrophoresis yana da sauƙin aiki, yanzu ana amfani dashi sosai a cikin bincike da gwajin furotin plasma, lipoprotein, glycoprotein, Alpha-fetoprotein, enzyme, polypeptide, nucleic acid da sauran kwayoyin halitta.
Shiri don Cellulose Acetate Membrane Electrophoresis
Kayan aikin da aka yi amfani da su sun haɗa da ƙarancin wutar lantarki, tankin electrophoresis a kwance. Kafofin watsa labaru masu goyan baya shine membrane acetate cellulose.
Reagents:
1.pH8.6 barbitol buffer bayani: Diethyl barbituric acid, Diethyl sodium pentobarbital;
2.Stain: Ponceau S, Trichloroacetic;
3.TBS / T ko PBS / T: 95% ethanol, glacial acetic acid;
4.Cleaning Magani: anhydrous ethanol, glacial acetic acid.
Beijing Liuyi Biotechnology Co., Ltd yana da tsarin balagagge don cellulose acetate membrane electrophoresis. Samfurin DYCP-38C wani ɗaki ne mai laushi don takarda da zamewa electrophoresis, da cellulose acetate membrane electrophoresis. Babban jikin yana gyaggyarawa, babu abin yabo. Samfurin samar da wutar lantarki DYY-2C, DYY-6C, DYY-6D, DYY-8C, DYY-10C da DYY-12 na iya ba da wutar lantarki don DYCP-38C. Gabaɗaya, abokin ciniki yana son zaɓin ƙirar DYY-6C azaman wutar lantarki don DYCP-38C.
A matsayin da ake bukata samfurin DYCP-38C electrophoresis tanki, Liuyi kuma samar da cellulose acetate membrane. Akwai nau'i-nau'i guda uku na membrane, 7 * 9cm, 2 * 8cm da 12 * 8cm, kuma za mu iya samar da girman girman cellulose acetate membrane kamar yadda bukatun ku.
Bayanan kula game da gwaji:
1.Load samfurin a kan m gefen: 1-2UL ya dace
2.Constant halin yanzu electrophoresis: ƙarfin halin yanzu 0.4 ~ 0.6m A/cm
3.pH8.6 barbitol buffer bayani: ƙarfin Ionic 0.06
4.Dyeing lokaci: 5 minutes isa
5.Preservation: sanya taswirar busassun elecrtophoresis a cikin bayani mai tsaftacewa na tsawon minti 10-15, sannan ku fitar da shi a kan gilashi mai tsabta, bayan ya bushe, zai zama taswirar fim mai haske.
Alamar Liuyi tana da tarihin fiye da shekaru 50 a kasar Sin kuma kamfanin na iya samar da samfuran barga da inganci a duk duniya.Ta hanyar ci gaban shekaru, ya cancanci zaɓinku!
Don ƙarin bayani game da mu, da fatan za a tuntuɓe mu ta imel[email protected], [email protected].
Lokacin aikawa: Afrilu-01-2022