Comet Assay (Single Cell Gel Electrophoresis, SCGE) fasaha ce mai mahimmanci kuma mai sauri da ake amfani da ita don gano lalacewar DNA da gyarawa a cikin sel guda ɗaya. Sunan "Comet Assay" ya fito ne daga siffa mai kama da tauraro mai wutsiya wanda ke bayyana a cikin sakamakon: tsakiya na tantanin halitta ya zama "kai," yayin da gutsuttssun DNA da suka lalace suna ƙaura, suna ƙirƙirar "wutsiya" mai kama da tauraro mai wutsiya.
Ka'ida
Ka'idar Comet Assay ta dogara ne akan ƙaura na gutsuttsuran DNA a cikin filin lantarki. DNA ɗin da ba ta dace ba ta kasance a cikin tsakiya ta tantanin halitta, yayin da DNA ɗin da ta lalace ko ɓaɓɓuke ke ƙaura zuwa ga anode, ta samar da “wutsiya” tauraro mai wutsiya. Tsawon tsayi da ƙarfin wutsiya suna daidai da girman lalacewar DNA.
Tsari
- Shiri CellKwayoyin da za a gwada ana haɗe su da agarose mai ƙarancin narkewa kuma ana yada su akan faifan microscope don samar da nau'in nau'i.
- Cell Lysis: Ana nutsar da nunin faifai a cikin maganin lysis don cire membrane cell da membrane na nukiliya, yana fallasa DNA.
- Electrophoresis: Ana sanya nunin faifai a cikin ɗakin electrophoresis a ƙarƙashin yanayin alkaline ko tsaka tsaki. Guguwar DNA da suka lalace suna ƙaura zuwa ingantacciyar wutar lantarki ƙarƙashin tasirin wutar lantarki.
- Tabo: Bayan electrophoresis, nunin faifai suna tabo da rini mai kyalli (misali, ethidium bromide) don ganin DNA.
- Ƙwararren Ƙwararru: Yin amfani da na'urar hangen nesa mai haske ko software na musamman, ana nazarin sifofin tauraro mai wutsiya, kuma ana auna sigogi kamar tsayin wutsiya da ƙarfi.
Hoto daga biorender
Binciken Bayanai
Ana kimanta sakamako daga Comet Assay bisa ga maɓalli da yawa:
- Tsawon Wutsiya: Yana wakiltar nisan da DNA ke yin ƙaura, yana nuna girman lalacewar DNA.
- Abun ciki na DNA wutsiya: Adadin DNA da ke ƙaura zuwa wutsiya, yawanci ana amfani da shi azaman ma'auni na lalacewar DNA.
- Lokacin wutsiya na Zaitun (OTM): Haɗa duka tsayin wutsiya da abun ciki na wutsiya don samar da ƙarin ma'auni na lalacewar DNA.
Aikace-aikace
- Nazarin Genotoxicity: Ana amfani da Comet Assay don tantance tasirin sinadarai, magunguna, da radiation akan DNA tantanin halitta, yana mai da shi kayan aiki mai mahimmanci don gwajin ƙwayoyin cuta.
- Environmental Toxicology: Yana taimakawa wajen nazarin tasirin gurɓataccen muhalli akan DNA na halitta, yana ba da haske game da amincin yanayin muhalli.
- Likita da Bincike na Clinical: Ana amfani da Comet Assay wajen nazarin hanyoyin gyaran DNA, ciwon daji, da sauran cututtuka masu alaƙa da DNA. Hakanan yana ƙididdige tasirin hanyoyin kwantar da cutar kansa kamar radiotherapy da chemotherapy akan DNA.
- Kimiyyar Abinci da Noma: Ana amfani da su don tantance amincin magungunan kashe qwari, kayan abinci, da sauran abubuwa, da kuma nazarin tasirin su mai guba a cikin nau'ikan dabbobi.
Amfani
- Babban Hankali: Iya gano ƙananan matakan lalacewar DNA.
- Aiki Mai Sauƙi: Dabarar ita ce madaidaiciya, yana sa ta dace da babban aikin nunawa.
- Fadin Application: Ana iya amfani da shi ga nau'ikan tantanin halitta daban-daban, ciki har da ƙwayoyin dabbobi da tsirrai.
- Kalubalen ƙididdigewa: Yayin samar da bayanai masu inganci akan lalacewar DNA, ƙididdigar ƙididdiga ta dogara sosai kan software da dabarun nazarin hoto.
- Yanayin Gwaji: Sakamako na iya rinjayar da abubuwa kamar lokacin electrophoresis da pH, suna buƙatar kulawa da hankali na yanayin gwaji.
Iyakance
The Comet Assay kayan aiki ne mai kima a cikin binciken ilimin halittu, kimiyyar muhalli, da haɓakar magunguna saboda sassaucinsa da babban azancinsa wajen gano lalacewar DNA da gyarawa. Beijing Liuyi Biotechnology Co. Ltd (Liuyi Biotechnology)yana ba da ɗakin electrophoresis na kwance don gwajin tauraron dan adam. Barka da zuwa tuntuɓar mu don tattaunawa game daComet Assayyarjejeniya.
Beijing Liuyi Biotechnology Co. Ltd (Liuyi Biotechnology) ya ƙware a masana'antar kayan aikin electrophoresis fiye da shekaru 50 tare da ƙungiyar ƙwararrun ƙwararrunmu da cibiyar R&D. Muna da amintaccen kuma cikakken layin samarwa daga ƙira zuwa dubawa, da ɗakunan ajiya, gami da tallafin talla. Babban samfuranmu sune Electrophoresis Cell (tanki / ɗakin), Samar da wutar lantarki ta Electrophoresis, Mai watsawa na LED mai haske, UV mai ɗaukar hoto, Gel Image & Analysis System da sauransu. Hakanan muna ba da kayan aikin lab kamar kayan aikin PCR, mahaɗar vortex da centrifuge don dakin gwaje-gwaje.
Idan kuna da wani shirin siyan samfuranmu, da fatan a yi shakka a tuntuɓe mu. Kuna iya aiko mana da sako ta imel[email protected]ko[email protected], ko kuma a kira mu a +86 15810650221 ko ƙara Whatsapp +86 15810650221, ko Wechat: 15810650221.
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Lokacin aikawa: Satumba-20-2024