DNA Gel Electrophoresis: Yin nazarin gutsuttsuran Halittu

DNA gel electrophoresis wata dabara ce ta ilmin halitta ta yau da kullun da ake amfani da ita don rarrabewa da nazarin gutsuttsuran DNA dangane da girmansu. Tsarin ya ƙunshi ɗora ɓangarorin DNA daban-daban akan gel ɗin da aka yi da agarose, carbohydrate da ake samu a cikin algae ja.

Ana shirya da jefar da gel na agarose

1. Narkar da agarose a cikin adadin da ya dace na buffer electrophoresis. An ƙaddara ƙaddamarwar gel ta hanyar ma'auni-zuwa-girma, kamar 1g na agarose a cikin 100.ml na buffer don 1% gel.
2. Zafi cakuda a cikin microwave, juya kwandon don tabbatar da cikakken rushewar agarose.
3. Ƙara ethidium bromide zuwa maganin gel zuwa ƙaddarar ƙarshe na 0.5mg/ ml. Ethidium bromide yana musaya tsakanin nau'ikan tushe na DNA na kusa kuma yana fitar da walƙiya orange ƙarƙashin hasken UV. Lura cewa ethidium bromide carcinogen ne, don haka sarrafa shi yana buƙatar matakan tsaro masu dacewa kamar sa safar hannu.
4. Sanya maganin gel a cikin wanka na ruwa don hana gel tire daga warping saboda yanayin zafi.
5. Sanya tsefe a cikin maganin gel don samar da rijiyoyin samfurin. Zaɓi combs masu dacewa da adadin samfurin DNA da za ku loda. Zuba maganin gel na agarose a cikintiren gelda kuma ƙyale shi ya ƙarfafa a zafin jiki.
6. Da zarar gel ya ƙarfafa, cire tsefe. Idan ba za ku yi amfani da gel nan da nan ba, kunsa shi a cikin filastik kunsa kuma adana shi a 4 ℃ har sai an buƙata.

Shirya da kuma gudanar da gel

Kafin fara electrophoresis, haɗa samfurin DNA tare da buffer loading. Makullin lodawa yawanci sau shida ya fi maida hankali kuma yana taimakawa samfurin nutsewa zuwa kasan rijiyoyi da bin diddigin motsi yayin electrophoresis.

Saita wutar lantarki zuwa ƙayyadadden ƙarfin lantarki.

Ƙara isassun buffer electrophoresis zuwa tankin gel don rufe saman gel.Tabbatardaidaitattun hanyoyin haɗin lantarki.

Loda samfurin DNA da alamomin nauyin kwayoyin a cikin rijiyoyin gel.

Kunna wutar lantarki don fara electrophoresis.

Kula da ɓangarorin DNA da aka ware

Bayan electrophoresis, kashe wutar lantarki kuma cire gel.

Yi amfani da tushen hasken UV don haskaka gel; Rubutun DNA za su bayyana a matsayin makada mai kyalli na orange.

Takaddun hoton gel don tantance gutsuttsuran DNA da suka rabu.

Bayan gwajin, tabbatar da zubar da kyau na gel da buffer electrophoresis, bin jagororin dakin gwaje-gwaje. Koyaushe sanya safar hannu yayin sarrafa gels da buffers masu ɗauke da ethidium bromide don kariya daga fallasa wannan abu mai haɗari.

Ana amfani da gel electrophoresis na DNA sosai a cikin ilimin halitta da bincike na kwayoyin halitta don kimanta girman kwayoyin halittar DNA, raba gutsuttsuran DNA, gano maye gurbi, da zanen DNA, a tsakanin sauran aikace-aikace. Dabarar gwaji ce mai sauƙi kuma mai tasiri wacce ke taimakawa wajen fahimtar abun da ke ciki da halayen samfuran DNA.

Beijing Liuyi Biotechnology Co.Ltd, ƙwararrun masana'anta da ke mai da hankali kan samfuran electrophoresis sama da shekaru 50, yana ba da tankuna masu yawa na electrophoresis (ɗakuna / sel) don gel electrophoresis, kamar tankunan lantarki na kwance (ɗakuna / sel) donDNAgel electrophoresis, da tankunan electrophoresis na tsaye (ɗakuna / sel) don gina jiki gel electrophoresis. A halin yanzu, yana kuma samar da nau'ikan wutar lantarki na lantarki daban-daban, mai duba akwatin duhu mai duhu da UV transilluminator. Kuna iya zaɓar tankunan lantarki na Liuyi na Beijing (ɗakuna/kwayoyin) don auna nauyin kwayoyin halitta na RNA da raba RNA masu girma dabam dabam don dakin binciken ku.

A nan za mubayar da shawararnau'in nau'in tankin electrophoresis na kwance (chamber/cell) samfurinSaukewa: DYCP-31DN don yin da kuma gudanar da gel.

Bayan gudanar da gel, zaku iya amfani da hoton gel ɗin mu & tsarin tsarin bincikeWD-9413don lura, nazari da ɗaukar hotuna don gel. Liuyi Biotech kuma yana ba da mai watsawa ta UV (UV Analyzer) don lura da gel. Muna da nau'in akwatin baƙar fata UV transilluminator (UV Analyzer).WD-9403A,9403C,WD-9403F, šaukuwa UV transilluminator (UV Analyzer) modelSaukewa: WD-9403Bda mai ɗaukar hoto na UV (UV Analyzer)WD-9403Edomin ka zaba.

Alamar Liuyi ta Beijing tana da tarihin sama da shekaru 50 a kasar Sin kuma kamfanin na iya samar da barga da kayayyaki masu inganci a duk duniya. Ta hanyar shekarun ci gaba, ya cancanci zaɓinku!

Yanzu muna neman abokan tarayya, duka OEM electrophoresis tank da masu rarraba suna maraba.

Idan kuna da wani shirin siyan samfuranmu, da fatan a yi shakka a tuntuɓe mu. Kuna iya aiko mana da sako ta imel[email protected]ko[email protected], ko kuma a kira mu a +86 15810650221 ko ƙara Whatsapp +86 15810650221, ko Wechat: 15810650221.