Gwaji don raba furotin na jini ta Cellulose Acetate Membrane Electrophoresis

Ka'ida

Cellulose acetate film electrophoresis shine hanyar electrophoresis ta amfani da fim din acetate cellulose azaman tallafi. Al'amarin da ɓangarorin da aka caje ke motsawa zuwa kishiyar lantarki a ƙarƙashin aikin filin lantarki ana kiransa electrophoresis. Tun da kowane furotin yana da takamaiman ma'anar isoelectric, idan an sanya furotin a cikin wani bayani tare da pH ƙasa da ma'anar isoelectric, furotin za a caje shi da kyau kuma ya matsa zuwa ga gurɓataccen lantarki. Akasin haka, yana motsawa zuwa sanda mai kyau. Saboda saurin kwayoyin sunadaran da ke motsawa a cikin wutar lantarki yana da alaƙa da cajin su, siffa da girman ƙwayoyin, sunadaran sunadaran za su iya raba su ta hanyar electrophoresis. Jini ya ƙunshi nau'ikan sunadaran, duk suna da maki isoelectric a pH7.5 ko ƙasa da haka. Lokacin da aka sanya serum a cikin pH 8.6 buffer don gudanar da electrophoresis, duk sunadaran sunadaran suna da caji mara kyau kuma za su matsa zuwa gefen tabbatacce a filin lantarki. Tunda sunadaran sunadarai daban-daban suna da caji daban-daban a pH ɗaya, ƙwayoyin ƙwayoyin ƙwayoyin cuta sun bambanta da girmansu, don haka saurin ƙaura ya bambanta, kuma ana raba su ta hanyar electrophoresis. Ana nuna maki isoelectric da ma'aunin kwayoyin halitta na furotin serum a cikin tebur da ke ƙasa:

Gwajin shine don raba sunadarai daban-daban a cikin jinin jini tare da cellulose acetate membrane (abbr. CAM) a matsayin kafofin watsa labaru. CAM wani nau'i ne na loos mai kumfaefim mai kyau uniform, kuma kauri ne 0.1mm-1.5mm, wanda yana da wani ruwa sha.

Materials, kayan aiki da reagents don CAM Electrophoresis

Misali:lafiyayyen jinin dan adam

Kayan aiki:wutar lantarki DYY-6C, tanki electrophoresis DYCP-38C, mafi girman samfurin loading WD-9404

Reagents

1) cellulose acetate membrane 7X9cm

2) PH 8.6 barbitol buffer bayani (ionic ƙarfi 0.05-0.09, shi ne 0.06tid lokaci.): Ɗauki 1,84g Diethyl barbituric acid, sa'an nan kuma 1.03g Diethyl sodium pentobarbital 1.03g Diethyl sodium pentobarbital, ƙara wani distilled ruwa don zafi ya narke, da kuma yin. har zuwa 1000 ml;

3) Tabo: Ponceau S 0.2g, Trichloroacetic 3g, ƙara 100ml distilled ruwa;

4) TBS / T ko PBS / T: 45ml 95% ethanol, 5ml glacial acetic acid, ƙara 50ml distilled ruwa;

5) Maganin tsaftacewa: 70ml anhydrous ethanol, 30ml glacial acetic acid.

Hanyar Gwajid

1) Shirya membrane: nutsar da membrane a cikin maganin buffer barbitol 30min-8h, kuma cire shi, cire karin bayani ta takarda mai sha.

2) Samfuran Loading: Rarraba gefen m da santsi na membrane, kuma yi alama a kan layi a nisan 1.5cm zuwa saman ƙarshen a gefen m. Load samfurori ta kayan aiki mafi girma na kayan aiki a gefen m. Lura: Ya kamata a ɗora samfuran a kan m gefen membrane. Bayan samfuran ruwan magani sun shiga cikin membrane, juya membrane, sanya gefen m (tare da samfurori) fuska zuwa tanki, kuma an sanya ƙarshen tare da samfurori a cikin wutar lantarki mara kyau.

3) Electrophoresis: Kunna wutar lantarki, 0.4～0.6m A/cm membrane, lokacin gudu shine 30-45 min. Bayan kunna electrophoresis, kashe wutar lantarki.

4) Tabo da tsabta: Cire membrane daga tanki, kuma a nutseit a cikin maganin rini na 5 min, sa'an nan kuma tsaftace shi a cikin maganin tsaftacewa akai-akai don sau 3-4 har sai launin baya ya bayyana. Ya kamata a nuna sunadaran jini a kan makada, kuma yawanci akwai yankuna biyar, suna samar da saman ƙarshen layi mai alama, Albumin, α1-gloulin, α2-gloulin, β-gloulin, γ-gloulin.

5) Kiyaye: sanya busassun elecrtophoresisbanda cikin bayani na tsaftacewa na minti 10-15, sa'an nan kuma fitar da shi kuma sanya shi a kan gilashi mai tsabta, bayan ya bushe, zai zama fim mai haske.band.

GwajiSakamako

Sakamakon rabuwa na samfurori na jini yana da kyau, kuma babu wani abin mamaki na band tailing. Maimaituwar sakamakon ya bambanta saboda hanyoyin gwajin da hanyoyin gwajin, kuma maimaitawa yana da yawa.

Kammalawa

Tsarin gano electrophoresis na asibiti mai sauri (Electrophoresis tankiDYCP-38C,tushen wutan lantarkiDYY-6C da mafi girman samfurin loading WD-9404) wanda muKamfanin Beijing Liuyi Biotechnology Co., Ltdya cika buƙatun gwaji,kumaSakamakon su ne reproducible, sauki da sauri, dace dakoyarwabincike.

Beijing LiuyiBiotechnology Co., Ltd ya ƙware a cikin kera samfuran Electophoresis don masana'antar kimiyyar rayuwa, wandayana da tarihin fiye da shekaru 50 a kasar Sin kuma kamfanin na iya samar da samfuran barga da inganci a duk faɗin duniya. Ta hanyar shekarun ci gaba, ya cancanci zaɓinku!

Yanzu muna neman abokan tarayya, duka OEM electrophoresis tank da masu rarraba suna maraba.

Don ƙarin bayani game da mu, da fatan za a tuntuɓe mu ta imel[email protected]ko[email protected].