Gwajin Haemoglobin Electrophoresis

Ƙa'idar Gwaji

Haemoglobin electrophoresis yana nufin ganowa da tabbatar da haemoglobins na al'ada daban-daban da marasa kyau.

Saboda daban-daban na caji da kuma isoelectric maki na daban-daban haemoglobin iri, a cikin wani pH buffer bayani, lokacin da isoelectric batu na haemoglobin ne m fiye da pH na buffer bayani, haemoglobin daukan wani mummunan cajin da kuma hijira zuwa ga anode a lokacin electrophoresis. Sabanin haka, haemoglobin tare da ingantaccen caji yana motsawa zuwa cathode.

Ƙarƙashin wani irin ƙarfin lantarki da kuma bayan takamaiman lokacin electrophoresis, haemoglobins tare da caji daban-daban da ma'aunin kwayoyin suna nuna kwatance daban-daban na ƙaura da sauri. Wannan yana ba da damar rarrabuwa na yankuna daban-daban, kuma za a iya yin nazari na launi mai launi ko electrophoretic a kan waɗannan yankuna don ƙididdige haemoglobin daban-daban. Hanyar da aka fi amfani da ita ita ce pH 8.6 cellulose acetate membrane electrophoresis.

A cikin cytoplasm, ƙungiyoyin ethylene glycol (CHOH-CHOH) da ke cikin glycogen ko abubuwan polysaccharide (irin su mucopolysaccharides, mucoproteins, glycoproteins, glycolipids, da dai sauransu) suna oxidized ta lokaci-lokaci acid kuma sun canza zuwa kungiyoyin aldehyde (CHO-CHO). Waɗannan ƙungiyoyin aldehyde suna haɗawa tare da ruwan sanyi-ja Schiff reagent mara launi, suna samar da rini mai shuɗi-ja wacce ke ajiye inda polysaccharides ke cikin tantanin halitta. An san wannan halayen da tabon acid-Schiff (PAS), wanda a da ake kira tabon glycogen.

Hanyar Gwaji

Kayayyaki:Cellulose acetatemembrane, electrophoresis na'ura(DYCP-38C da wutar lantarki DYY-6C), Babban Samfurin Loading Tool(pipette), spectrophotometer, launi mai launi, buffers.

Buffer:

(1) pH 8.6 TEB Buffer: Auna 10.29 g Tris, 0.6 g EDTA, 3.2 g boric acid, kuma ƙara distilled ruwa zuwa 1000 ml.

(2) Borate Buffer: Auna 6.87 g borax da 5.56 g boric acid, kuma ƙara distilled ruwa zuwa 1000 ml.

Tsari:

Pgyara Maganin Haemoglobin

Ɗauki 3 ml na jini mai ɗauke da heparin ko sodium citrate a matsayin maganin rigakafi. Centrifuge a 2000 rpm na minti 10 kuma jefar da plasma. A wanke jajayen ƙwayoyin jinin sau uku tare da saline na physiological (750 rpm, 5 minutes centrifugation kowane lokaci). Centrifuge a 2200 rpm na minti 10 kuma jefar da supernatant. Ƙara daidaitaccen adadin ruwa mai narkewa, sannan ƙara sau 0.5 ƙarar carbon tetrachloride. Girgizawa da ƙarfi na tsawon mintuna 5, sannan a ɗaure a 2200 rpm na minti 10 don tattara maganin Hb na sama don amfani daga baya.

Jiƙan Membrane

Yanke membrane acetate cellulose cikin tube masu auna 3 cm × 8 cm. Jiƙa su a cikin pH 8.6 TEB buffer har sai an cika su, sannan a cire kuma a bushe da takarda tace.

Tabo

Yi amfani da pipette don tabo 10 μl na maganin haemoglobin a tsaye a kan membrane acetate cellulose (m gefen), kimanin 1.5 cm daga gefen.

Electrophoresis

Zuba maganin buffer na borate cikin ɗakin electrophoresis. Sanya membrane acetate cellulose tare da gefen tabo a ƙarshen cathode na ɗakin. Gasa a 200V na minti 30.

Haske

Yanke yankunan HbA da HbA2, sanya su a cikin bututun gwaji daban, kuma ƙara 15 ml da 3 ml na ruwa mai narkewa, bi da bi. A hankali girgiza don cire haemoglobin gaba daya, sannan a hade.

Launi mai launi

Zero abin sha ta amfani da ruwa mai tsafta don maganin haɓakawa kuma auna abin sha a 415 nm.

Lissafi

HbA2(%) = Cire bututun HbA2 / (Sharwar bututun HbA × 5 + Shakar HbA2 tube) × 100%

Lissafin Sakamakon Gwaji

Range Range don pH 8.6 TEB Buffer Cellulose Acetate Electrophoresis: HbA> 95%, HbA2 1% -3.1%

Bayanan kula

Lokacin Electrophoresis bai kamata ya yi tsayi da yawa ba. Membran acetate cellulose kada ya bushe a lokacin electrophoresis. Dakatar da electrophoresis lokacin da HbA da HbA2 suka rabu. Tsawon electrophoresis na iya haifar da yaduwar bandeji da blurring.

Ka guji amfani da samfur da yawa. Ruwan haemoglobin da ya wuce kima na iya haifar da rabuwar bandeji ko rashin isassun tabo, yana haifar da haɓakar matakan HbA na ƙarya.

Hana gurɓatar membrane acetate cellulose tare da sunadarai.

Na yanzu bai kamata ya yi tsayi da yawa ba; in ba haka ba, haemoglobin bandeji ba zai iya rabu.

Koyaushe haɗa samfura daga daidaikun mutane na yau da kullun da sananan haemoglobins mara kyau a matsayin masu sarrafawa.

Liuyi Biotechnology ya kera tankin electrophoresis na ƙwararrun don haemoglobin electrophoresis wanda shine samfurin.Saukewa: DYCP-38Ccellulose acetate membrane electrophoresis tank, kuma akwai nau'i biyu na samar da wutar lantarki na electrophoresis samuwa ga cellulose acetate membrane electrophoresis tank.DAY-2CkumaDYY-6Ctushen wutan lantarki.

A halin yanzu, Liuyi Biotechnology na Beijing yana samar da membrane acetate cellulose ga abokan ciniki, kuma ana iya daidaita girman membrane acetate cellulose. Barka da zuwa tambaye mu samfurori da ƙarin bayani.

Alamar Liuyi ta Beijing tana da tarihin sama da shekaru 50 a kasar Sin kuma kamfanin na iya samar da barga da kayayyaki masu inganci a duk duniya. Ta hanyar shekarun ci gaba, ya cancanci zaɓinku!

Yanzu muna neman abokan tarayya, duka OEM electrophoresis tank da masu rarraba suna maraba.

Idan kuna da wani shirin siyan samfuranmu, da fatan a yi shakka a tuntuɓe mu. Kuna iya aiko mana da sako ta imel[email protected]ko[email protected], ko kuma a kira mu a +86 15810650221 ko ƙara Whatsapp +86 15810650221, ko Wechat: 15810650221