Agatashigel electrophoresis
Agarose gel electrophoresis hanya ce ta gel electrophoresis da aka yi amfani da ita a cikin ilmin halitta, ilmin halitta, kwayoyin halitta, da sunadarai na asibiti don raba gauraye da yawa na macromolecules kamar DNA koRNA. Wannan yana samuwa ta hanyar motsa ƙwayoyin nucleic acid da ba su da caji ta hanyar matrix agarose tare daelectrophoresis.
Properties na agarose gel
Agarose gel shine matrix mai girman nau'i uku da aka kafa na kwayoyin agarose na helical a cikin nau'i-nau'i masu yawa waɗanda aka haɗa su zuwa sassa uku tare da tashoshi da pores ta hanyar da kwayoyin halitta zasu iya wucewa. Tsarin 3-D yana riƙe da haɗin gwiwar hydrogen kuma saboda haka za'a iya rushewa. ta hanyar dumama komawa zuwa yanayin ruwa. Yanayin narkewa ya bambanta da zafin jiki na gelling, dangane da tushen, agarose gel yana da zafin jiki na 35.-42 ° C da zafin jiki na narkewa na 85-95 °C. Ana samun ƙarancin narkewa da ƙarancin gelling agaroses da aka yi ta hanyar gyare-gyaren sinadarai.
Abũbuwan amfãni da rashin amfani na agarose gel electrophoresis
Babban fa'idar fasaha ta agarose gel electrophoresis ita ce ana iya sarrafa ta cikin sauƙi kuma kwayar halittar DNA da ake amfani da ita azaman samfurin kuma za'a iya dawo da ita ba tare da cutar da ita ba a ƙarshen aikin. Saboda gel na agarose bai yi ba't ƙunshi sulfate da ƙungiyar carboxyl, ba zai haifar da electroosmosis na agar ba. Tare da tsarin daidaituwa da girman girman pore, gel na agarose yana da kyauraba manyan kwayoyin halittar DNA, kamar hadaddun enzyme, nucleic acid da ƙwayoyin cuta. Agarose gel bai dace ba't sha hasken UV, don haka ana iya lalata shi kai tsaye ko bayan gelling ya zama fim, yana da jujjuyawar thermally kuma.
Hakanan akwai wasu rashin amfani na fasahar agarose gel electrophoresis. Agarose gel yana sauƙi karye, don haka yawancin ba zai iya zama ƙasa da ƙasa ba, zai kashe ƙarin agarose.Gel na agarose yana da wuyar ajiyewa saboda yana da sauƙi don gurɓatar da ƙwayoyin cuta, kuma yakamata a shirya shi kafin amfani da shi. Idan aka kwatanta da dabarar electrophoresis na PAGE, ingancin sieve na kwayoyin ya ragu, kuma Yankin electrophoresis ya ragu.
A kwanceelectrophoresistsarin ga agarose gel
Sai dai agarose da buffer, gwajin agarose gel electrophoresis yana buƙatar tsarin electrophoresis a kwance da ƙananan-wutar lantarki electrophoresis samar da wutar lantarki.Liuyi Biotechnology na Beijing na iya ba da tsarin lantarki a kwance da kuma electrophoresis samar da wutar lantarki, kamar modelSaukewa: DYCP-31BN, Saukewa: DYCP-31CN domina kwance electrophoresis tsarin kumaDYY-6C don samar da wutar lantarki.
Dogara a kan nau'ikan gel daban-dabanbukata ta Agrose gel electrophoresis gwaji, daban-daban masu girma dabam na kwance electrophoresis tsarin za a iya bayar da Beijing Liuyi Biotechnology. Model DYCP-31 jerin,skamarSaukewa: DYCP-31BN, Saukewa: DYCP-31CN, Saukewa: DYCP-31DN, Saukewa: DYCP-31E, DYCP-32B, kumaSaukewa: DYCP-32Csuna da nau'i daban-daban na gel tray, daga simintin ƙaramin gel ɗin gel zuwa babban girman gel, za su iya saduwa da gwajin ku.bukatun.
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Lokacin aikawa: Afrilu-16-2022