Anan za mu bayyana yadda ake yin agarose gel electrophoresis ta mai bincikenmu a dakin binciken Liuyi Biotech.
Kafin gwajin, muna buƙatar bincika na'urori, reagents, da sauran kayan gwaji da kayan aikin da muke buƙata.
Shirye-shiryen na'urorin gwaji da kayan aiki
Na'urorin don agarose gel electrophoresis
A kwance electrophoresis cell (tanki / jam'iyya), matsakaici da ƙananan ƙarfin lantarki electrophoresis ikon samar, gel image & bincike tsarin.
Beijing Liuyi Biotechnology Co., Ltd (Liuyi Biotech) yana ba da nau'ikan nau'ikan nau'ikan tantanin halitta na kwance (tanki/ɗaki) da samar da wutar lantarki gami da tsarin takaddun gel. Samfuran jerin samfuran DYCP-31 sune na electrophoresis, kuma samfuran jerin DYY sune wadatar wutar lantarki. Samfuran jerin WD-9413 sune tsarin takaddun gel. Daga kananan gel size 60 × 60mm zuwa m gel 250 × 250mm, za mu iya saduwa da daban-daban bukatun ga gel size. SamfurinSaukewa: DYCP-32Ciya sa gel zuwa girman 250 × 250mm. TheDYY-6Cshine wutar lantarkinmu. Yana goyan bayan fitarwa na 400V, 400mA, 240W, wanda shine samfuranmu na yau da kullun da abokan cinikinmu ke amfani da su. WD-9413Gel Image & Analysis System Ana amfani da shi don nazari da bincike don gel, fina-finai, da ɓoyayyen bayan gwajin electrophoresis. Na'ura ce ta asali tare da tushen hasken ultraviolet don gani da ɗaukar hotunan gels ɗin da aka lalata da rini mai kyalli kamar ethidium bromide, kuma tare da tushen haske mai haske don gani da ɗaukar hoto da gels ɗin da aka lalata da rini kamar coomassie m shuɗi.
Reagents don agarose gel electrophoresis
1.Matsakaici: agarose gel
2.The buffer: TAE (Tris-acetate, EDTA, glacial acetic acid) da kuma TBE (Tris-borate, EDTA, boric acid).
3.The loading buffer: 6 × DNA Loading Buffer (Na musamman ga DNA samfurin: EDAT, glycerine, Xylene cyanol da bromophenol blue)
Mai Rini
Rini mai kyalli kamar EB, Gelred, goldview, GenGreen, GenView, SYBRGreen
TheAbubuwan amfani
Sterilization pipette tukwici (10μL), pipette tukwici (200μL), pipette tukwici (1000μL), 200μL \ 500μL \ 1.5ml centrifuge tube.
Alamar DNA
An shirya bisa ga nauyin kwayoyin da za a gwada.
Gel yana gudana kuma ya lura
Da farko, muna buƙatar shirya gel na agarose. Mahimmancin agarose a cikin gel zai dogara ne akan girman ɓangarorin DNA da za a rabu, tare da mafi yawan gels tsakanin 0.5% -2%. Shan muSaukewa: DYCP-31DNmisali, Za ka iya jefa kananan gel, m gel , dogon gel da square gel bisa ga bukatun da gwajin. Zaɓi tiren gel ɗin da kuke buƙata, sannan ku saka tsefe, sannan Zuba gel ɗin agarose mai zafi a cikin na'urar simintin gel ɗin.
Sa'an nan, bayan gel ya zama mai ƙarfi, cire tsefe a hankali kuma a hankali, sa'an nan kuma sanya gel tire a cikin tanki mai buffer. Zuba maganin buffer a cikin tankin buffer kuma sanya duk gel ɗin a nutsar da shi a cikin buffer. Load da samfurori a cikin rijiyoyin tare da daidaitattun pipette. Haɗa daSaukewa: DYCP-31DNtare da wutar lantarki na electrophoresis daidai, kuma saita sigogi don gudanar da gel.
Yi tunanin gutsuwar DNA ɗin ku a cikin mai watsawa UV.
ABayan gudanar da gel, zaku iya amfani da hoton gel ɗin mu & tsarin tsarin bincikeWD-9413don lura, nazari da ɗaukar hotuna don gel. Liuyi Biotech kuma yana ba da mai watsawa ta UV (UV Analyzer) don lura da gel. Muna da nau'in akwatin akwatin UV trsamfurin ansilluminator (UV Analyzer).WD-9403A, 9403C, WD-9403F, šaukuwa UV transilluminator (UV Analyzer) modelSaukewa: WD-9403Bda mai ɗaukar hoto na UV (UV Analyzer)WD-9403Edomin ka zaba.
Hoton gel post electrophoresis
Alamar Liuyi tana da fiye da shekaru 50 na tarihi a kasar Sin kuma kamfanin na iya samar da samfuran barga da inganci a duk duniya. Ta hanyar shekarun ci gaba, ya cancanci zaɓinku!
Don ƙarin bayani game da mu, da fatan za a tuntuɓe mu ta imel[email protected], [email protected].
Lokacin aikawa: Afrilu-29-2022