Gabatarwa
Gel electrophoresis wata hanya ce ta asali a cikin ilimin halittar kwayoyin halitta, ana amfani da ita sosai don rabuwa da sunadarai, acid nucleic, da sauran macromolecules. Ingantacciyar kulawar ƙarar samfurin, ƙarfin lantarki, da lokacin electrophoresis yana da mahimmanci don samun ingantacciyar sakamako mai ƙima.Abokin aikinmu na lab yayi tayimafi kyawun ayyuka don sarrafa waɗannan sigogi yayin SDS-PAGE gel electrophoresis.
Liuyi Biotechnology gel electrophoresis kayayyakin
Girman Samfura: Tabbatar da daidaito
Lokacin yin SDS-PAGE electrophoresis, ƙimar samfurin shine maɓalli mai mahimmanci wanda zai iya tasiri sosai ga ƙudurin sakamakon ku. Gabaɗaya ana ba da shawarar ɗaukar 10µL na jimlar furotin kowace rijiya. Don tabbatar da daidaito da hana yaduwar samfurin tsakanin rijiyoyin da ke kusa, yana da mahimmanci a ɗora nauyin daidaitaccen buffer 1x a cikin kowane rijiyoyin da ba kowa. Wannan rigakafin yana taimakawa wajen hana yaduwar samfurori zuwa hanyoyin makwabta, wanda zai iya faruwa idan an bar rijiyar babu kowa.
Kafin loda samfuran ku, koyaushe fara da ƙara alamar ma'aunin ƙwayar cuta zuwa rijiya ɗaya. Wannan yana ba da damar sauƙin gano girman furotin bayan electrophoresis.
Ikon wutar lantarki: Daidaita Gudun Gudun da ƙuduri
Wutar lantarki da ake amfani da ita a lokacin electrophoresis kai tsaye yana rinjayar duka saurin da samfurori ke yin ƙaura ta gel da ƙudurin rabuwa. Don SDS-PAGE, yana da kyau a fara da ƙananan ƙarfin lantarki na kusan 80V. Wannan ƙananan wutar lantarki na farko yana ba da damar samfurori don yin ƙaura a hankali kuma a ko'ina, suna mai da su a cikin maɗaukaki mai kaifi yayin da suke shiga gel mai raba.
Da zarar samfurori sun shiga cikin gel mai rarrabawa, ana iya ƙara ƙarfin lantarki zuwa 120V. Wannan babban ƙarfin lantarki yana haɓaka ƙaura, yana tabbatar da cewa sunadaran sun rabu da kyau gwargwadon nauyin kwayoyin su. Yana da mahimmanci don saka idanu da ci gaban bromophenol blue dye gaban, wanda ke nuna kammala electrophoresis. Don gels tare da maida hankali na 10-12%, 80-90 mintuna yawanci ya isa; duk da haka, don 15% gels, kuna iya buƙatar ƙara lokacin gudu kaɗan.
Gudanar da Lokaci: Sanin Lokacin Tsayawa
Lokaci wani muhimmin abu ne a cikin gel electrophoresis. Gudun gel na dogon lokaci ko gajeren lokaci na iya haifar da rabuwa mara kyau. Gudun hijira na bromophenol blue dye ne mai amfani mai nuna alama: lokacin da ya kai kasan gel, yawanci lokaci ne don dakatar da gudu. Don daidaitattun gels, kamar 10-12%, tsawon lokacin electrophoresis na kusan mintuna 80-90 yawanci ya isa. Don mafi girman gels, kamar 15%, ya kamata a tsawaita lokacin gudu don tabbatar da cikakken rabuwa da sunadaran.
Gudanar da Buffer: Sake amfani da Shirye Shirye-shirye
Ana iya sake amfani da buffer Electrophoresis sau 1-2, ya danganta da takamaiman yanayin dakin gwaje-gwaje. Koyaya, don kyakkyawan sakamako, ana ba da shawarar shirya sabon buffer 10x kuma a tsoma shi kafin amfani. Wannan yana tabbatar da cewa buffer yana kula da ingancinsa, yana haifar da ingantaccen sakamako na electrophoresis.
Liuyi Biotechnology gel electrophoresis kayayyakin
Ta hanyar sarrafa ƙarar samfurin a hankali, ƙarfin lantarki, da lokacin electrophoresis, zaku iya haɓaka daidaito da haɓaka sakamakon gel electrophoresis ɗinku sosai. Aiwatar da waɗannan ingantattun ayyuka a cikin aikin dakin gwaje-gwajen ku zai taimaka muku samun ƙarin fayyace kuma ƙarin nau'ikan makada, yana haifar da ingantattun bayanai don bincike na ƙasa.
Idan kuna da ƙarin hanyoyi masu kyau don haɓaka gwajin gel electrophoresis, maraba don tattaunawa tare da mu!
Beijing Liuyi Biotechnology Co. Ltd (Liuyi Biotechnology) ya ƙware a masana'antar kayan aikin electrophoresis fiye da shekaru 50 tare da ƙungiyar ƙwararrun ƙwararrunmu da cibiyar R&D. Muna da amintaccen kuma cikakken layin samarwa daga ƙira zuwa dubawa, da ɗakunan ajiya, gami da tallafin talla. Babban samfuranmu sune Electrophoresis Cell (tanki / ɗakin), Samar da wutar lantarki ta Electrophoresis, Mai watsawa na LED mai haske, UV mai ɗaukar hoto, Gel Image & Analysis System da sauransu. Hakanan muna ba da kayan aikin lab kamar kayan aikin PCR, mahaɗar vortex da centrifuge don dakin gwaje-gwaje.
Idan kuna da wani shirin siyan samfuranmu, da fatan a yi shakka a tuntuɓe mu. Kuna iya aiko mana da sako ta imel[email protected]ko[email protected], ko kuma a kira mu a +86 15810650221 ko ƙara Whatsapp +86 15810650221, ko Wechat: 15810650221.
Da fatan za a bincika lambar QR don ƙara akan Whatsapp ko WeChat.
Lokacin aikawa: Agusta-20-2024