A cikin yanayin da ke ci gaba da bunkasar ilimin kwayoyin halitta, Polymerase Chain Reaction (PCR) da Gel Electrophoresis sun fito a matsayin dabarun ginshiƙan da ke sauƙaƙe bincike da sarrafa DNA. Waɗannan hanyoyin ba kawai suna da alaƙa da bincike ba amma kuma suna da aikace-aikace da yawa a cikin bincike, kimiyyar bincike, da fasahar kere-kere.
Hoto daga gidan yanar gizon biology4alevel
PCR wata dabara ce ta juyin juya hali da Kary Mullis ta kirkira a cikin 1983, wanda ke baiwa masana kimiyya damar fadada takamaiman sashin DNA a sarari. Wannan tsari yana farawa ne tare da raguwar DNA, inda DNA mai madauri biyu ta kasance mai zafi zuwa kusan 94 ° C, yana sa ta rabu gida biyu guda ɗaya. Ana biye da wannan tare da annealing, inda abubuwan farko - gajerun jeri na nucleotides - suna ɗaure ga jerin madaidaitan akan DNA guda ɗaya a ƙananan zafin jiki (yawanci kusan 55 ° C). A ƙarshe, lokacin tsawaita yana faruwa a 72 ° C, inda enzyme DNA polymerase ke haɗa sabon layin DNA ta ƙara nucleotides zuwa abubuwan farko. Ana maimaita wannan sake zagayowar sau 20-40, wanda ke haifar da miliyoyin kwafi na jerin DNA da aka yi niyya.
Da zarar an haɓaka DNA ɗin, ana amfani da Gel Electrophoresis don rarrabewa da bincika samfuran PCR. Wannan dabarar ta ƙunshi ƙaura na gutsuttsuran DNA ta hanyar matrix gel agarose a ƙarƙashin tasirin wutar lantarki. Kwayoyin DNA suna da mummunan caji saboda kashin bayansu na phosphate, kuma suna ƙaura zuwa ga ingantacciyar lantarki. Gel yana aiki azaman sieve, yana ƙyale ƙananan sassan DNA don motsawa da sauri fiye da manyan. Sakamakon haka, an raba gutsuttsuran DNA bisa girmansu, tare da ganuwa daban-daban a ƙarƙashin hasken ultraviolet bayan tabo da rini kamar ethidium bromide.
Beijing LIUYIGel Electrophoresis Products
Haɗin PCR da Gel Electrophoresis yana da ƙarfi a cikin aikace-aikace da yawa. A cikin binciken likita, ana amfani da PCR don gano kasancewar ƙwayoyin cuta, maye gurbi, ko takamaiman jerin DNA masu alaƙa da cuta. Gel Electrophoresis sannan yana ba da damar gani da kuma tabbatar da waɗannan ɓangarorin DNA masu ƙarfi. A cikin kimiyyar bincike, waɗannan dabarun suna da mahimmanci don ɗaukar yatsan yatsa na DNA, inda suke taimakawa daidaita samfuran DNA daga wuraren aikata laifuka tare da waɗanda ake zargi.
Beijing Liuyi Biotechnology Co. Ltd (Liuyi Biotechnology) ya ƙware a masana'antar kayan aikin electrophoresis fiye da shekaru 50 tare da ƙungiyar ƙwararrun ƙwararrunmu da cibiyar R&D. Muna da amintaccen kuma cikakken layin samarwa daga ƙira zuwa dubawa, da ɗakunan ajiya, gami da tallafin talla. Babban samfuranmu sune Electrophoresis Cell (tanki / ɗakin), Samar da wutar lantarki ta Electrophoresis, Mai watsawa na LED mai haske, UV mai ɗaukar hoto, Gel Image & Analysis System da sauransu. Hakanan muna ba da kayan aikin lab kamar kayan aikin PCR, mahaɗar vortex da centrifuge don dakin gwaje-gwaje.
Beijing LIUYIGel Electrophoresis Products
Idan kuna da wani shirin siyan samfuranmu, da fatan a yi shakka a tuntuɓe mu. Kuna iya aiko mana da sako ta imel[email protected]ko[email protected], ko kuma a kira mu a +86 15810650221 ko ƙara Whatsapp +86 15810650221, ko Wechat: 15810650221.
Da fatan za a bincika lambar QR don ƙara akan Whatsapp ko WeChat.
Lokacin aikawa: Agusta-15-2024