Ana Shirya Gel Agarose Don Electrophoresis

Shiri na Agarose Gel don Electrophoresis

Lura: Koyaushe sanya safofin hannu masu yuwuwa!

Umarnin mataki-mataki

Yin Auna Agarose Foda:use auna takarda da ma'aunin lantarki don auna 0.3g na foda agarose (dangane da tsarin 30ml).

Ana shirya TBST Buffer:pgyara 30ml na 1x TBST buffer a cikin 100ml Erlenmeyer flask.

Narkar da Foda Agarose:pfoda na agarose a cikin buffer TBST kuma girgiza sosai.Saka su a cikizuwa microwave da zafi (yawanci don 50 seconds, an rufe shi da foil aluminum) har sai an narkar da shi gaba daya.

Sanyaya da Ƙara Nukiliya:use safar hannu don cire cakuda daga microwave kuma bari ya dan yi sanyi a cikin ruwan sanyi har sai ya dumi (kimanin 60 ° C). Ƙara 2µl na nuclease (Eb madadin) yayin girgiza don haɗuwa sosai.

Ana Shirya Gel Mold:

• Cjingina da bushe dagel tray da gel simintin na'urarna electrophoresis tanki.
•  Pyadin da aka saka geltirea cikin tanki na ciki kuma saka tsefe a wuri mai mahimmanci.
• Mix da maganin agarose gel, sanyaya zuwa kimanin 65 ° C, kuma a hankali zuba shi a kantiren gela cikingel simintin na'urar, yada shi a hankali har sai ya samar da madaidaicin gel a kan farantin gilashi.
• Bada shi ya zauna a dakin da zafin jiki har sai gel ɗin ya ƙarfafa gaba ɗaya.
• A hankali cire tsefet a tsaye kuma cire tef ɗin.
• Sanya geltirea cikin tanki na electrophoresis.

Muhimmi: Tabbatar cewa babu kumfa mai iska a wurin tsefewar haƙoran. Ya kamata saman ruwan ya zama santsi ba tare da ripples ba.

Gudun Gel

Load da Gel

Bayan da gel ɗin ya ƙarfafa, sanya shi a cikin tanki na electrophoresis kuma ku zubar da buffer electrophoresis har sai gel ya nutse.

Ana shirya Samfurori

• Ɗauki alamar alama da buffer daga firji.
• Ƙara 6µl na buffer loading zuwa samfuran kuma haxa da kyau.
• Yin amfani da micropipette, sannu a hankali ɗora samfuran a cikin manyan rijiyoyin gel (ku yi hankali kada ku huda gel ɗin kuma kada ku watsar da duka ƙarar don guje wa kumfa).
• Loda alamar a cikin kowane ƙananan rijiyoyin (tuna da matsayinsa).

Fara Electrophoresis

• Rufe tanki na electrophoresis kuma fara electrophoresis nan da nan bayan loda gel ɗin.
• Saita ƙarfin lantarki zuwa 60-100V. Samfuran za su yi ƙaura daga mummunan lantarki (baƙar fata) zuwa ingantaccen lantarki (ja).
• Babban ƙarfin lantarki yana rage tasirin rabuwa mai tasiri na gel agarose.
• Dakatar da electrophoresis lokacin da launin shudi na bromophenol ya kai kusan 1cm daga gefen ƙasa na farantin gel.

Duban Sakamako

Bayan an raba makada, dakatar da electrophoresis, fitar da gel, kuma gano kai tsaye da daukar hoto.

Yi amfani da tsarin hoton gel don ɗaukar hoto da lura da makada (duba idan akwai wasu makada don alamar ko samfurori).

Bayan samun taswirar band ɗin gel ɗin ku, nemo alamar. Dangane da alamar, zaku iya ƙayyade maƙallan manufa!

Beijing Liuyi Biotechnology Co. Ltd (Liuyi Biotechnology) an ƙera samfuran electrophoresis sama da shekaru 50. Mun gabatar da mu kwance electrophoresis tsarin a nan donagarose gelelectrophoresis.Zabia kwance electrophoresis tsarindaga Beijing liuyi don gwajin ku na gel electrophoresis don taimakawa bincikenku a masana'antar fasahar kere kere.

Muna ba da samfura daban-daban na tankunan lantarki na kwance don yin simintin gyare-gyare da ƙaramiagarosegels zuwa babbaagarosegels

Jagoran Zaɓi don Rukunin Gel Electrophoresis na kwance a kwance

Beijing Liuyi Biotechnology Co. Ltd (Liuyi Biotechnology) ya ƙware a masana'antar kayan aikin electrophoresis fiye da shekaru 50 tare da ƙungiyar ƙwararrun ƙwararrunmu da cibiyar R&D. Muna da amintaccen kuma cikakken layin samarwa daga ƙira zuwa dubawa, da ɗakunan ajiya, gami da tallafin talla. Babban samfuranmu sune Electrophoresis Cell (tanki / ɗakin), Samar da wutar lantarki ta Electrophoresis, Mai watsawa na LED mai haske, UV mai ɗaukar hoto, Gel Image & Analysis System da sauransu. Hakanan muna ba da kayan aikin lab kamar kayan aikin PCR, mahaɗar vortex da centrifuge don dakin gwaje-gwaje.

Idan kuna da wani shirin siyan samfuranmu, da fatan a yi shakka a tuntuɓe mu. Kuna iya aiko mana da sako ta imel[email protected]ko[email protected], ko kuma a kira mu a +86 15810650221 ko ƙara Whatsapp +86 15810650221, ko Wechat: 15810650221.

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