Electrophoresis wata dabara ce da ake amfani da ita don raba kwayoyin halittu dangane da girmansu da cajin su ta amfani da filin lantarki. Ana amfani da shi sosai a cikin ilimin kimiyyar halittu don dalilai daban-daban, kama daga binciken DNA zuwa tsarkakewar furotin. Anan, zamu bincika ka'idar electrophoresis da aikace-aikacen sa iri-iri.
Ka'idar Electrophoresis
Electrophoresis ya dogara ne akan motsin da aka caje a filin lantarki. Saitin asali ya haɗa da sanya samfurin (wanda ke ɗauke da cajin biomolecules) akan gel ko a cikin bayani, da amfani da wutar lantarki. Kwayoyin halittu suna yin ƙaura ta tsakiya a farashi daban-daban dangane da cajin su da girmansu, yana haifar da rabuwa.
Nau'in Electrophoresis
1. Gel Electrophoresis
Agarose Gel Electrophoresis: Yana raba DNA da gutsuttsura RNA dangane da girman.
Polyacrylamide Gel Electrophoresis (PAGE): Yana magance sunadaran dangane da girma da caji.
2. Capillary Electrophoresis
Yana amfani da kunkuntar capillaries don rabuwa, yana ba da damar yin bincike cikin sauri na DNA, RNA, da sunadarai.
Aikace-aikace a cikin Kimiyyar Halittu
1. Binciken DNA
Genotyping: Yana gano bambance-bambancen kwayoyin halitta (misali, SNPs) masu alaƙa da cututtuka.
Tsarin DNA: Yana ƙayyade tsari na nucleotides a cikin kwayoyin DNA.
Binciken Juzu'i na DNA: Girman gutsuwar DNA don aikace-aikace a cikin ilimin halitta.
2. Binciken RNA
RNA Electrophoresis: Yana raba kwayoyin RNA don nazarin maganganun kwayoyin halitta da amincin RNA.
3. Binciken Protein
SDS-PAGE (Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis): Rarraba sunadaran dangane da girman.
2D Electrophoresis: Haɗa mayar da hankali kan isoelectric da SDS-PAGE don raba sunadarai dangane da ma'ana da girman isoelectric.
4. Tsarkakewa
Preparative Electrophoresis: Yana tsarkake biomolecules (misali, sunadarai) bisa caji da girma.
5. Aikace-aikacen asibiti
Haemoglobin Electrophoresis: Yana bincikar cututtukan haemoglobinopathies (misali, cutar sikila).
Serum Protein Electrophoresis: Yana gano rashin daidaituwa a cikin sunadaran jini.
6. Aikace-aikace na Forensic
Bayanan Halitta na DNA: Ya dace da samfuran DNA don binciken bincike.
Amfanin Electrophoresis
Babban Ƙaddamarwa: Yana raba kwayoyin halitta bisa girman da caji tare da madaidaicin madaidaici.
Ƙarfafawa: Ana amfani da DNA, RNA, sunadarai, da sauran ƙwayoyin halitta da aka caje.
Ƙididdigar ƙididdigewa: Yana auna yawan adadin kwayoyin halitta bisa ƙarfin bandeji.
Beijing Liuyi Biotechnology Co. Ltd (Liuyi Biotechnology) ya ƙware a masana'antar kayan aikin electrophoresis fiye da shekaru 50 tare da ƙungiyar ƙwararrun ƙwararrunmu da cibiyar R&D. Muna da amintaccen kuma cikakken layin samarwa daga ƙira zuwa dubawa, da ɗakunan ajiya, gami da tallafin talla. Babban samfuranmu sune Electrophoresis Cell (tanki / ɗakin), Samar da wutar lantarki ta Electrophoresis, Mai watsawa na LED mai haske, UV mai ɗaukar hoto, Gel Image & Analysis System da sauransu. Hakanan muna ba da kayan aikin lab kamar kayan aikin PCR, mahaɗar vortex da centrifuge don dakin gwaje-gwaje.
Idan kuna da wani shirin siyan samfuranmu, da fatan a yi shakka a tuntuɓe mu. Kuna iya aiko mana da sako ta imel[email protected]ko[email protected], ko kuma a kira mu a +86 15810650221 ko ƙara Whatsapp +86 15810650221, ko Wechat: 15810650221.
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Lokacin aikawa: Mayu-28-2024