Mun raba wasu batutuwa na gama gari game da makada na electrophoresis a baya, kuma mu'Ina so in raba wasu abubuwan ban mamaki na polyacrylamide gel electrophoresis a wani gefen. Mutaƙaita waɗannan batutuwa don abokan cinikinmu' duba don gano dalilai da zuwasami ingantaccen sakamako kuma inganta ingancin rabuwar furotin.
Gel ba polymerized Yiwuwa ba
Dalilan su ne:
(a) Rashin isasshen tsarkin monomer, yana buƙatar recrystallization.
(b) Narkar da oxygen a cikin maganin gel yana hana gel polymerization. Ya kamata a yi amfani da famfo mai tasiri mai tasiri.
(c) Ammonium persulfate ba shi da inganci ko bai isa ba a yawa. Shirya sabo ko amfani da wani nau'in ammonium persulfate tare da maida hankali sosai.
Tshi gel ya riga ya zama polymerized ba tare da ƙara ruwan Layer ba.
Dalilan su ne:
(a) Sanya maganin gel kafin ƙara mai kara kuzari don rage yawan adadin polymerization.
(b) Rage adadin TEMED ko ammonium persulfate da ake amfani da shi.
(c) Gaggauta aikin.
Gel yana zamewa daga ɗakin electrophoresis bayan polymerization
Wannan yana da wuyar faruwa a cikin ƙananan gels masu hankali. Ɗayan bayani shine a nannade membrane na dialysis a kusa da kasan bututu ko amfani da tushe mai tushe na polypropylene.
Ba a gano samfurin ba bayan electrophoresis
Dalilan su ne:
(a) Rashin isassun adadin samfurin. Ƙara adadin samfurin.
(b) Kaddarorin maganin tabo ko maida hankali ko lokacin lalata ba su isa ba. Sauya maganin tabo kuma ƙara yawan taro da lokacin lalata.
(c) Samfurin ya yi iyo a yayin lodawa. Ƙara yawa na maganin samfurin kuma rike da kulawa.
(d) Rarraba gel taro yana da yawa, kuma samfurin bai shiga gel ba. Daidaita taro na gel daidai.
(e) Rarraba gel taro ya yi ƙasa da ƙasa, kuma samfurin da aka electrophoresed daga cikin rabuwa gel. Daidaita taro na gel da mafi kyawun yanayin electrophoresis.
(f) Idan samfurin RNA ne, yana iya ƙunsar sunadaran da ke haifar da babban hadaddun da ke toshe ramukan gel. Cire furotin sosai daga samfurin RNA.
(g) Samfurin yana ƙunshe da enzymes waɗanda ke haɓaka samfurin, kuma samfurin ya ƙasƙanta yayin electrophoresis. Tsarkake samfurin sosai.
Kamfanin Beijing Liuyi Biotechnology Company Ltd yana kera kewayon samfuran electrophoresis waɗanda za su iya taimakawa wajen warware matsalolin da wataƙila za mu iya saduwa da su a gwajin electrophoresis ɗin mu. An kafa kamfanin ne a shekarar 1970. Mallakar kasa ce kuma ta samar da injin walda lantarki da na'urar auna wutar lantarki a wancan lokacin. Tun 1979, Beijing Liuyi Biotechnology Company Ltd ya fara kera samfuran electrophoresis. Yanzu kamfaninis daya daga cikin manyanmai kera kayan aikin dakin gwaje-gwaje da na'urorin kimiyya da ke birnin Beijing, kasar Sin. Its alamar kasuwanci"LIUYI” ya shahara a kasar Sin a wannan fanni.
Kayayyakin kamfanin sun hada da kayan aikin dakin gwaje-gwaje da dama, ciki har da kwancen nucleic acid electrophoresis tank, a tsaye protein electrophoresis tanki / naúrar, baki-akwatinnau'in UV analyzer,Gel Document Tracking Hoto Analyzer, da wutar lantarki na lantarki. Ana amfani da waɗannan samfuran sosai a cibiyoyin bincike, jami'o'i, da masana'antu daban-daban, kamar su magunguna, fasahar kere-kere, da kula da muhalli.Kamfanin shine ISO9001 & ISO13485 bokan kamfani kuma yana da takaddun CE.
Akwaiiri-iri iri-iria tsayeelectrophoresis tankuna donfurotin electrophoresisdon bincike da gano samfuran furotin ta hanyar polyacrylamide gel electrophoresis,kumaHar ila yau, don auna samfurori' nauyin kwayoyin halitta, samfurori masu tsarkakewa da shirya samfurori.Waɗannan samfuran duk ana maraba da sukasuwannin cikin gida da na ketare.
Samar da wutar lantarki na electrophoresis shine muhimmin bangarenelectrophoresis tsarin, samar da tsayayyen tushen wutar lantarki don fitar da tsarin rabuwa.Ityawanci yana ba da wutar lantarki akai-akai ko na yau da kullun zuwa tsarin electrophoresis, dangane da ƙayyadaddun ƙa'idar gwaji. Hakanan yana ba mai amfani damar daidaita ƙarfin lantarki ko fitarwa na yanzu, da sauran sigogi kamar lokaci da zafin jiki, don haɓaka yanayin rabuwa don takamaiman gwaji.
Fko lura da gel, za ka iya zabar UV Transilluminator WD-9403 jerin kerarre ta Beijing Liuyi Biotechnology.A UV transilluminator kayan aikin dakin gwaje-gwaje ne da ake amfani dashi don gani da kuma tantance samfuran DNA, RNA, da furotin. Yana aiki ta hanyar haskaka samfurori tare da hasken UV, wanda ke haifar da samfurori zuwa haske kuma ya zama bayyane. Akwai da yawa model na UV transilluminatormiƙa muku da mu. WD-9403A na musamman ne don lura da furotin electrophoresis, kuma WD-9403F ana amfani dashi don lura da DNA da furotin electrophoresis.
Waɗannan jerin samfuran suna iya aiki daga gel ɗin simintin simintin gyare-gyare zuwa gel mai lura bisa ga buƙatun gwajin ku.Bari mu san bukatun ku, OEM, ODM da masu rarraba suna maraba.We zai yi iya ƙoƙarinmu don ba ku samfuranmu da sabis ɗinmu.
Idan kuna da wani shirin siyan samfuranmu, da fatan a yi shakka a tuntuɓe mu. Kuna iya aiko mana da sako ta imel[email protected]ko[email protected], ko kuma a kira mu a +86 15810650221 ko ƙara Whatsapp +86 15810650221, ko Wechat: 15810650221.
Lokacin aikawa: Mayu-23-2023