Kuna da wata matsala wajen shirya gel na agarose?Bari mu biyo bayaMasanin binciken mu a cikin shirya gel.
Tsarin shirye-shiryen gelrose gel ya ƙunshi matakai masu zuwa:
Yin Auna Agarose Foda
Yi la'akari da adadin da ake buƙata na foda agarose bisa ga ƙaddarar da ake so don gwajin ku. Matsakaicin adadin agarose na yau da kullun yana daga 0.5% zuwa 3%. Ana amfani da maɗaukaki mafi girma don rarraba ƙananan ƙwayoyin DNA, yayin da ƙananan ƙididdiga don ƙananan ƙwayoyin cuta.
Ana Shirya Maganin Buffer
Ƙara foda na agarose zuwa madaidaicin buffer electrophoresis, kamar 1 × TAE ko 1× TBE. Ya kamata ƙarar buffer ya dace da ƙarar gel ɗin da ake buƙata don gwajin ku.
Warkar da Agarose
Gasa cakuda agarose da buffer har sai agarose ya narke gaba daya. Ana iya yin wannan ta amfani da microwave ko farantin zafi. Juya maganin a lokaci-lokaci don hana shi tafasa. Maganin agarose ya kamata ya bayyana ba tare da wani ɓangarorin bayyane ba.
Cooling Maganin Agarose
Bada maganin agarose mai zafi ya yi sanyi zuwa kusan 50-60 ° C. Dama maganin yayin aikin sanyaya don hana haɓakar da bai kai ba.
Ƙara Tabon Nucleic Acid (Na zaɓi)
Idan kuna son ganin DNA ko RNA a cikin gel, zaku iya ƙara tabon acid nucleic, kamar GelRed ko ethidium bromide, a wannan matakin. Lokacin sarrafa waɗannan tabo, sanya safar hannu kuma yi taka tsantsan, saboda suna iya zama mai guba.
Yin jifa da Gel
Zuba maganin agarose da aka sanyaya a cikin injin gel na electrophoresis da aka shirya. Saka tsefe don ƙirƙirar rijiyoyin samfurin, tabbatar da cewa tsefe yana da tsaro kuma an rarraba maganin daidai a cikin ƙirar.
Gel Solidification
Bada gel ɗin don ƙarfafawa a cikin zafin jiki, wanda yawanci yana ɗaukar mintuna 20-30 dangane da taro da kauri na gel.
Rmotsa Comb
Da zarar gel ɗin ya yi ƙarfi sosai, cire tsefe a hankali don bayyana rijiyoyin samfurin. Sanya gel tare da mold a cikin ɗakin electrophoresis kuma rufe shi da adadin da ya dace na buffer electrophoresis, tabbatar da cewa gel ɗin ya cika.
Ana shirya don Electrophoresis
Bayan an shirya gel ɗin, ɗora samfuran ku a cikin rijiyoyin, kuma ku ci gaba da gwajin electrophoresis.
Idan kuna da wasu batutuwa tare da shirye-shiryen gel, jin daɗin tuntuɓar mu. Muna da ƙwararrun ƙwararrun ƙwararrun ƙwararrun ƙwararru don sadarwa tare da ku.
Muna farin cikin raba wasu manyan labarai: sanannen tankin mu na DYCP-31DN kwance electrophoresis a halin yanzu yana kan haɓakawa., Tuntube mu don ƙarin bayani yanzu!
DYCP-31DN a kwance electrophoresis tanki
Beijing Liuyi Biotechnology Co. Ltd (Liuyi Biotechnology) ya ƙware a masana'antar kayan aikin electrophoresis fiye da shekaru 50 tare da ƙungiyar ƙwararrun ƙwararrunmu da cibiyar R&D. Muna da amintaccen kuma cikakken layin samarwa daga ƙira zuwa dubawa, da ɗakunan ajiya, gami da tallafin talla. Babban samfuranmu sune Electrophoresis Cell (tanki / ɗakin), Samar da wutar lantarki ta Electrophoresis, Mai watsawa na LED mai haske, UV mai ɗaukar hoto, Gel Image & Analysis System da sauransu. Hakanan muna ba da kayan aikin lab kamar kayan aikin PCR, mahaɗar vortex da centrifuge don dakin gwaje-gwaje.
Idan kuna da wani shirin siyan samfuranmu, da fatan a yi shakka a tuntuɓe mu. Kuna iya aiko mana da sako ta imel[email protected]ko[email protected], ko kuma a kira mu a +86 15810650221 ko ƙara Whatsapp +86 15810650221, ko Wechat: 15810650221.
Da fatan za a bincika lambar QR don ƙara akan Whatsapp ko WeChat.
Lokacin aikawa: Oktoba-15-2024