Menene tsarin hawan keke na thermal a cikin PCR?

Polymerase Chain Reaction (PCR) wata dabara ce ta ilimin halitta da ake amfani da ita don haɓaka takamaiman gutsuttsuran DNA. Ana iya la'akari da shi nau'i na musamman na kwafin DNA a waje da kwayoyin halitta. Babban fasalin PCR shine ikonsa na haɓaka adadin DNA sosai.

Bayanin Zagayowar Amsar Sarkar Polymerase

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Menene tsarin hawan keke na thermal a cikin PCR?

PCR ya ƙunshi matakai na asali guda uku:

1. Denaturation of Template DNA: Ana ƙona samfurin DNA ɗin zuwa kusa da 93°C na wani ɗan lokaci, yana haifar da DNA mai ɗaure biyu zuwa ɓangarorin guda ɗaya, yana shirya shi don ɗaure firam a cikin zagaye na gaba na gaba.

2. Annealing of Template DNA and Primers: Bayan denaturation, ana saukar da zafin jiki zuwa kusa da 55°C, yana barin firamare su ɗaure ga madaidaitan jeri akan DNA samfuri mai ɗaci ɗaya.

3. Extension of Primers: A 72 ° C, DNA template-primer complexes yana fuskantar tsawo tare da taimakon DNA polymerase (kamar Taq DNA polymerase), ta amfani da dNTPs a matsayin substrates don haɗa sabon madaidaicin madaidaicin bisa ga DNA samfuri.

Injin PCR na Beijing LIUYI

Ta hanyar maimaituwa da tsawaitawa, cirewa, da tsawaitawa, ana samar da ƙarin “matsalolin kwafi masu ra'ayin mazan jiya, waɗanda za su iya zama samfuri don zagayawa masu zuwa. Kowane sake zagayowar yana ɗaukar kusan mintuna 2 zuwa 4, yana ƙyale jigon da aka yi niyya don haɓaka miliyoyin sau a cikin sa'o'i 2 zuwa 3.

Beijing Liuyi Biotechnology Co. Ltd (Liuyi Biotechnology) ya ƙware a masana'antar kayan aikin electrophoresis fiye da shekaru 50 tare da ƙungiyar ƙwararrun ƙwararrunmu da cibiyar R&D. Muna da amintaccen kuma cikakken layin samarwa daga ƙira zuwa dubawa, da ɗakunan ajiya, gami da tallafin talla. Babban samfuranmu sune Electrophoresis Cell (tanki / ɗakin), Samar da wutar lantarki ta Electrophoresis, Mai watsawa na LED mai haske, UV mai ɗaukar hoto, Gel Image & Analysis System da sauransu. Hakanan muna ba da kayan aikin lab kamar kayan aikin PCR, mahaɗar vortex da centrifuge don dakin gwaje-gwaje.

Idan kuna da wani shirin siyan samfuranmu, da fatan a yi shakka a tuntuɓe mu. Kuna iya aiko mana da sako ta imel[email protected]ko[email protected], ko kuma a kira mu a +86 15810650221 ko ƙara Whatsapp +86 15810650221, ko Wechat: 15810650221.

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