PCR Thermal Cycler WD-9402D
Ƙayyadaddun bayanai
| Samfura | Saukewa: WD-9402D |
| Iyawa | 96 × 0.2ml |
| Tube | 0.2ml tube, 8 tube, Half skirt96 rijiyoyin farantin, Babu siket 96 rijiyoyin farantin |
| Girman martani | 5-100 ul |
| Yanayin Zazzabi | 0-105 ℃ |
| MAX. Matsakaicin Ragewa | 5 ℃/s |
| Daidaituwa | ≤± 0.2℃ |
| Daidaito | ≤± 0.1℃ |
| Nuni Resolution | 0.1 ℃ |
| Kula da Zazzabi | Block/Tube |
| Rage Rage Daidaitacce | 0.01-5 ℃ |
| Yanayin zafi. Rage | 30-105 ℃ |
| Nau'in Gradient | Al'ada Gradient |
| Yaduwar Gradient | 1-42 ℃ |
| Zafin Rufin Zafi | 30-115 ℃ |
| Yawan Shirye-shirye | 20000 + (USB FLASH) |
| Max. No. na Mataki | 40 |
| Max. No. na Zagaye | 200 |
| Ƙaruwa / Rage Lokaci | 1 seconds - 600 seconds |
| Zazzabi / Ragewa | 0.1-10.0 ℃ |
| Aikin Dakata | Ee |
| Kariyar Bayanai ta atomatik | Ee |
| Tsaya a 4 ℃ | Har abada |
| Ayyukan taɓawa | Ee |
| Dogon aikin PCR | Ee |
| Harshe | Turanci |
| Software na Kwamfuta | Ee |
| Wayar hannu APP | Ee |
| LCD | 10.1 inch, 1280 × 800 pels |
| Sadarwa | USB2.0, WIFI |
| Girma | 385mm × 270mm × 255mm (L×W×H) |
| Nauyi | 10kg |
| Tushen wutan lantarki | 100-240VAC, 50/60Hz, 600W |
Bayani
Mai hawan keke na thermal yana aiki ta akai-akai dumama da sanyaya cakuduwar da ke ɗauke da samfurin DNA ko RNA, abubuwan farko, da nucleotides. Ana sarrafa hawan keken zafin jiki daidai don cimma maƙasudin rarrabuwar kawuna, cirewa, da tsawaita matakan tsarin PCR.
Yawanci, mai hawan keke na thermal yana da shinge mai ɗauke da rijiyoyi da yawa ko bututu inda aka sanya cakudawar amsawa, kuma ana sarrafa zafin jiki a kowace rijiya da kansa. Ana zafi da sanyaya toshe ta amfani da sinadarin Peltier ko wani tsarin dumama da sanyaya.
Yawancin masu hawan keke na thermal suna da haɗin haɗin kai mai amfani wanda ke ba mai amfani damar tsarawa da daidaita ma'aunin hawan keke, kamar zafin zafi, lokacin tsawaitawa, da adadin hawan keke. Hakanan suna iya samun nuni don saka idanu kan ci gaban halayen, kuma wasu ƙila za su ba da fasalulluka na ci gaba kamar sarrafa zafin jiki na gradient, saitunan toshe da yawa, da sa ido da sarrafawa na nesa.
Aikace-aikace
Genome cloning; Asymmetric PCR shirye-shiryen DNA guda ɗaya don jerin DNA; PCR mai juyayi don ƙayyade yankunan DNA da ba a sani ba; PCR (RT-PCR). Don gano matakin maganganun kwayoyin halitta a cikin sel, da adadin ƙwayar cutar RNA da cloning kai tsaye na cDNA tare da takamaiman kwayoyin halitta; saurin haɓakawa na cDNA ƙare; gano maganganun kwayoyin halitta; za a iya amfani da shi don gano cututtukan ƙwayoyin cuta da ƙwayoyin cuta; ganewar cututtuka na kwayoyin halitta; ganewar asali na ciwace-ciwacen daji; bincike na likita kamar shaida ta zahiri, ba za a iya amfani da shi a cikin binciken asibiti na likitanci ba.
Fitattu
• Babban yawan dumama da sanyaya, max. Matsakaicin haɓaka 8 ℃/s;
• Sake kunnawa ta atomatik bayan gazawar wutar lantarki. Lokacin da aka dawo da wutar lantarki zai iya ci gaba da gudanar da shirin da ba a gama ba;
• Ayyukan incubation da sauri danna-ɗaya na iya biyan buƙatun gwaji kamar denaturation, yankan enzyme / mahaɗin enzyme da ELISA;
• Za a iya saita yanayin zafin murfi mai zafi da yanayin aikin murfi don saduwa da buƙatun gwaji daban-daban;
• Yana amfani da ƙayyadaddun ƙayyadaddun kekuna na zafin jiki na Peltier na tsawon rayuwa;
• Anodized aluminum module tare da aikin injiniya ƙarfafawa, wanda ke riƙe da saurin tafiyar da zafi kuma yana da isasshen juriya na lalata;
• Matsakaicin saurin zafin jiki, tare da matsakaicin matsakaicin matsakaicin 5 ° C/s, adana lokacin gwaji mai mahimmanci;
• Murfin zafi mai salo mai daidaita matsi, wanda za'a iya rufe shi tam tare da mataki ɗaya kuma yana iya daidaitawa zuwa tsayin bututu daban-daban;
• Tsarin iska na gaba-da-baya, yana ba da damar sanya injina gefe da gefe;
• Yana amfani da tsarin aiki na Android, wanda ya dace da allon taɓawa mai ƙarfi mai inci 10.1, tare da ƙirar menu mai hoto mai hoto, yana yin aiki mai sauƙi;
• Samfuran fayilolin shirin daidaitattun 11 da aka gina a ciki, wanda zai iya gyara fayilolin da ake buƙata da sauri;
• Nuna ainihin ci gaban shirin da sauran lokacin, tallafawa tsakiyar shirye-shiryen kayan aikin PCR;
• Ayyukan incubation mai sauri-maballin, saduwa da bukatun gwaje-gwaje kamar denaturation, enzyme narkewa / ligation, da ELISA;
• Za'a iya saita yanayin zafin murfin zafi da yanayin aiki mai zafi don saduwa da buƙatun gwaji daban-daban;
• Kariyar kashe wutar lantarki ta atomatik, aiwatar da hawan keke ta atomatik bayan an dawo da wutar lantarki, tabbatar da aiki mai aminci a cikin tsarin haɓakawa;
• Kebul na USB yana goyan bayan ajiyar bayanai/dawowar PCR ta amfani da kebul na USB kuma yana iya amfani da linzamin kwamfuta na USB don sarrafa kayan aikin PCR;
• Yana goyan bayan sabunta software ta USB da LAN;
• Tsarin WIFI da aka gina a ciki, ba da damar kwamfuta ko wayar hannu don sarrafa kayan aikin PCR da yawa a lokaci guda ta hanyar haɗin yanar gizo;
• Yana goyan bayan sanarwar imel lokacin da shirin gwaji ya cika.
FAQ
Tambaya: Menene mai hawan keke na thermal?
A: Mai hawan keken thermal na'urar dakin gwaje-gwaje ce da ake amfani da ita don haɓaka jerin DNA ko RNA ta hanyar sarrafa sarkar polymerase (PCR). Yana aiki ta hawan keke ta jerin sauye-sauyen zafin jiki, yana ba da damar haɓaka takamaiman jerin DNA.
Tambaya: Menene manyan abubuwan da ke cikin injin zazzagewa?
A: Babban abubuwan da ke cikin injin zazzagewa sun haɗa da toshe mai dumama, na'urar sanyaya thermoelectric, na'urori masu auna zafin jiki, microprocessor, da kwamiti mai kulawa.
Tambaya: Ta yaya mai hawan keke na thermal ke aiki?
A: Mai hawan keke na thermal yana aiki ta hanyar dumama da sanyaya samfuran DNA a cikin jerin yanayin yanayin zafi. Tsarin keken keke ya ƙunshi denaturation, annealing, da matakan tsawo, kowanne tare da takamaiman zafin jiki da tsawon lokaci. Waɗannan kewayon suna ba da damar haɓaka takamaiman jerin DNA ta hanyar amsawar sarkar polymerase (PCR).
Tambaya: Menene muhimman abubuwan da za a yi la'akari da su lokacin zabar mai hawan zafi? A: Wasu muhimman fasalulluka da za a yi la'akari da su lokacin zabar mai hawan keke sun haɗa da adadin rijiyoyi ko bututun amsawa, kewayon zafin jiki da saurin gudu, daidaito da daidaituwar yanayin zafin jiki, da ƙirar mai amfani da damar software.
Tambaya: Yaya kuke kula da na'urar hawan zafi?
A: Don kula da mai hawan keke, yana da mahimmanci a kai a kai a tsaftace shingen dumama da bututun amsawa, bincika lalacewa da tsagewa akan abubuwan da aka gyara, da daidaita na'urori masu auna zafin jiki don tabbatar da daidaitaccen kulawar zafin jiki. Hakanan yana da mahimmanci a bi umarnin masana'anta don kulawa da gyara na yau da kullun.
Tambaya: Wadanne matakai ne gama gari na warware matsalar mai tuka keken thermal?
A: Wasu matakan magance matsalar gama gari don mai hawan zafi sun haɗa da duba sako-sako da abubuwan da suka lalace, tabbatar da madaidaicin zafin jiki da saitunan lokaci, da gwada bututun amsawa ko faranti don lalacewa ko lalacewa. Hakanan yana da mahimmanci a koma zuwa umarnin masana'anta don takamaiman matakan warware matsala da mafita.
