PCR Thermal Cycler WD-9402M
Ƙayyadaddun bayanai
| Samfura | WD-9402M |
| Iyawa | 96 × 0.2ml |
| Tube | 96x0.2ml (PCR farantin ba tare da / Semi siket), 12x8x0.2ml tube, 8x12x0.2ml tube, 0.2ml tubes (tsawo 20 ~ 23mm) |
| Toshe Rage Zazzabi | 0-105 ℃ |
| Toshe Daidaiton Zazzabi | ± 0.2 ℃ |
| Toshe Uniformity na Zazzabi | ± 0.5 ℃ |
| Adadin Haɓakawa (matsakaici) | 4 ℃ |
| Ƙimar sanyi (matsakaici) | 3 ℃ |
| Kula da Zazzabi | Block/Tube |
| Yanayin zafi. Rage | 30-105 ℃ |
| Matsakaicin Yawan Zafafawa | 5 ℃/s |
| Matsakaicin Matsayin Sanyaya 4.5℃/S | 4.5 ℃/s |
| Girman Saitin Taɗi | Max. 42 ℃ |
| Daidaitaccen Zazzabi na Gradient | ± 0.3 ℃ |
| daidaiton nunin zafin jiki | 0.1 ℃ |
| Zazzabi Rufe Zazzabi | 30 ℃ ~ 110 ℃ |
| Rufe mai dumama ta atomatik | Kashe ta atomatik lokacin da samfurin ƙasa da 30 ℃ ko shirin ya ƙare |
| Ƙayyadaddun lokaci / Ragewa | -599~599 S don Dogon PCR |
| Zazzabi / Ragewa | -9.9 ~ 9.9 ℃ don PCR Touchdown |
| Mai ƙidayar lokaci | 1s~59min59sec/ Mara iyaka |
| An adana shirye-shirye | 10000+ |
| Max.Cycles | 99 |
| Matsakaicin Matakai | 30 |
| Aikin Dakata | Ee |
| Ayyukan taɓawa | Ee |
| Dogon aikin PCR | Ee |
| Harshe | Turanci |
| Ayyukan Dakatawar Shirin | Ee |
| 16 ℃ Aikin Rike Zazzabi | Mara iyaka |
| Matsayin aiki na ainihi | An nuna rubutun-hoto |
| Sadarwa | Kebul na USB 2.0 |
| Girma | 200mm × 300mm × 170mm (W×D × H) |
| Nauyi | 4.5kg |
| Tushen wutan lantarki | 100-240VAC, 50/60Hz, 600W |
Bayani
Mai hawan keke na thermal yana aiki ta akai-akai dumama da sanyaya cakuduwar da ke ɗauke da samfurin DNA ko RNA, abubuwan farko, da nucleotides. Ana sarrafa hawan keken zafin jiki daidai don cimma maƙasudin rarrabuwar kawuna, cirewa, da tsawaita matakan tsarin PCR.
Yawanci, mai hawan keke na thermal yana da shinge mai ɗauke da rijiyoyi da yawa ko bututu inda aka sanya cakudawar amsawa, kuma ana sarrafa zafin jiki a kowace rijiya da kansa. Ana zafi da sanyaya toshe ta amfani da sinadarin Peltier ko wani tsarin dumama da sanyaya.
Yawancin masu hawan keke na thermal suna da haɗin haɗin kai mai amfani wanda ke ba mai amfani damar tsarawa da daidaita ma'aunin hawan keke, kamar zafin zafi, lokacin tsawaitawa, da adadin hawan keke. Hakanan suna iya samun nuni don saka idanu kan ci gaban halayen, kuma wasu ƙila za su ba da fasalulluka na ci gaba kamar sarrafa zafin jiki na gradient, saitunan toshe da yawa, da sa ido da sarrafawa na nesa.
Aikace-aikace
Polymerase Chain Reaction (PCR) wata dabara ce ta ilimin halitta da ake amfani da ita don aikace-aikace daban-daban. Wasu aikace-aikacen gama gari na PCR sun haɗa da:
Ƙwaƙwalwar DNA: Babban manufar PCR shine haɓaka takamaiman jerin DNA. Wannan yana da mahimmanci don samun isassun adadin DNA don ƙarin bincike ko gwaji.
Gwajin Halittar Halitta: Ana amfani da PCR sosai a gwajin kwayoyin halitta don gano takamaiman alamomin kwayoyin halitta ko maye gurbi masu alaƙa da cututtuka. Yana da mahimmanci don dalilai na bincike da kuma nazarin abubuwan da ke tattare da kwayoyin halitta.
DNA Cloning: Ana amfani da PCR don samar da adadi mai yawa na takamaiman guntun DNA, wanda za'a iya haɗa shi zuwa cikin vector don ƙarin magudi ko bincike.
Binciken DNA na Forensic: PCR yana da mahimmanci a cikin kimiyyar bincike don haɓaka samfuran DNA na mintuna da aka samu daga wuraren aikata laifuka. Yana taimakawa wajen gano daidaikun mutane da kafa dangantakar kwayoyin halitta.
Gano Kwayoyin cuta: Ana amfani da PCR don gano ƙwayoyin cuta na ƙwayoyin cuta a cikin samfuran asibiti ko samfuran muhalli. Yana ba da damar gano saurin gano cututtukan cututtuka.
PCR mai ƙididdigewa (qPCR ko Real-Time PCR): qPCR yana ba da damar ƙididdige adadin DNA yayin aikin haɓakawa. Ana amfani da shi don auna matakan maganganun kwayoyin halitta, gano nauyin ƙwayoyin cuta, da ƙididdige adadin takamaiman jerin DNA.
Nazarin Juyin Halitta: Ana amfani da PCR a cikin nazarin nazarin bambance-bambancen kwayoyin halitta a cikin al'umma, dangantakar juyin halitta, da kuma nazarin halittu.
Binciken DNA na Muhalli (eDNA): Ana amfani da PCR don gano kasancewar takamaiman kwayoyin halitta a cikin samfuran muhalli, suna ba da gudummawa ga bambancin halittu da nazarin muhalli.
Injiniyan Halitta: PCR kayan aiki ne mai mahimmanci a cikin injiniyan kwayoyin halitta don gabatar da takamaiman jerin DNA cikin kwayoyin halitta. Ana amfani da shi wajen ƙirƙirar kwayoyin halitta (GMOs).
Shirye-shiryen Laburaren Jeri: PCR tana da hannu a cikin shirye-shiryen dakunan karatu na DNA don fasahohin jeri na gaba. Yana taimakawa haɓaka guntun DNA don aikace-aikacen jeri na ƙasa.
Mutagenesis-Directed Site: Ana amfani da PCR don gabatar da takamaiman maye gurbi cikin jerin DNA, ƙyale masu bincike suyi nazarin tasirin sauye-sauyen ƙwayoyin halitta.
Buga yatsa na DNA: Ana amfani da PCR a cikin dabarun zanen yatsan DNA don gano mutum ɗaya, gwajin uba, da kafa alaƙar halittu.
Siffar
• Kyakkyawar bayyanar, m size, da m tsari.
• An sanye shi da babban aiki, mai shiru axial-flow fan don tsarin aiki mai natsuwa.
• Yana da faffadan aikin gradient na 30℃, yana ba da damar inganta yanayin gwaji don saduwa da ƙaƙƙarfan buƙatun gwaji.
• 5-inch high-definition color touchscreen for ilhama da kuma sauki aiki, kunna kokarin kokarin gyara, ceto, da kuma gudanar da shirye-shirye.
•Tsarin aiki na masana'antu, yana sauƙaƙe aiki mai ci gaba da rashin kuskure 7x24.
• Canja wurin bayanai cikin sauri zuwa kebul na filasha don sauƙin shirin madadin, haɓaka ƙarfin ajiyar bayanai.
•Babban fasahar sanyaya semiconductor da fasaha na sarrafa zafin jiki na PID na musamman suna haɓaka aikin gabaɗaya zuwa sabon tsayi: daidaitattun sarrafa zafin jiki, saurin dumama da yanayin sanyaya, da yanayin yanayin yanayin rarraba iri ɗaya.
FAQ
Tambaya: Menene mai hawan keke na thermal?
A: Mai hawan keken thermal na'urar dakin gwaje-gwaje ce da ake amfani da ita don haɓaka jerin DNA ko RNA ta hanyar sarrafa sarkar polymerase (PCR). Yana aiki ta hawan keke ta jerin sauye-sauyen zafin jiki, yana ba da damar haɓaka takamaiman jerin DNA.
Tambaya: Menene manyan abubuwan da ke cikin injin zazzagewa?
A: Babban abubuwan da ke cikin injin zazzagewa sun haɗa da toshe mai dumama, na'urar sanyaya thermoelectric, na'urori masu auna zafin jiki, microprocessor, da kwamiti mai kulawa.
Tambaya: Ta yaya mai hawan keke na thermal ke aiki?
A: Mai hawan keke na thermal yana aiki ta hanyar dumama da sanyaya samfuran DNA a cikin jerin yanayin yanayin zafi. Tsarin keken keke ya ƙunshi denaturation, annealing, da matakan tsawo, kowanne tare da takamaiman zafin jiki da tsawon lokaci. Waɗannan kewayon suna ba da damar haɓaka takamaiman jerin DNA ta hanyar amsawar sarkar polymerase (PCR).
Tambaya: Menene muhimman abubuwan da za a yi la'akari da su lokacin zabar mai hawan zafi?
A: Wasu muhimman fasalulluka da za a yi la'akari da su lokacin zabar mai hawan keke sun haɗa da adadin rijiyoyi ko bututun amsawa, kewayon zafin jiki da saurin gudu, daidaito da daidaituwar yanayin zafin jiki, da ƙirar mai amfani da damar software.
Tambaya: Yaya kuke kula da na'urar hawan zafi?
A: Don kula da mai hawan keke, yana da mahimmanci a kai a kai a tsaftace shingen dumama da bututun amsawa, bincika lalacewa da tsagewa akan abubuwan da aka gyara, da daidaita na'urori masu auna zafin jiki don tabbatar da daidaitaccen kulawar zafin jiki. Hakanan yana da mahimmanci a bi umarnin masana'anta don kulawa da gyara na yau da kullun.
Tambaya: Wadanne matakai ne gama gari na warware matsalar mai tuka keken thermal?
A: Wasu matakan magance matsalar gama gari don mai hawan zafi sun haɗa da duba sako-sako da abubuwan da suka lalace, tabbatar da madaidaicin zafin jiki da saitunan lokaci, da gwada bututun amsawa ko faranti don lalacewa ko lalacewa. Hakanan yana da mahimmanci a koma zuwa umarnin masana'anta don takamaiman matakan warware matsala da mafita.
