Filin Pulsed Gel Electrophoresis CHEF Mapper A1
Ƙayyadaddun bayanai
| Samfura | CHEF Mapper A1 |
| Girman Wutar Lantarki | 0.5V/cm zuwa 9.6V/cm, an ƙaru da 0.1V/cm |
| Matsakaicin Yanzu | 0.5A |
| Matsakaicin Wutar Lantarki | 350V |
| Kwangilar bugun jini | ± 120° |
| Lokaci Gradient | Litattafai |
| Lokacin Canjawa | 50ms zuwa 18h |
| Matsakaicin Lokacin Gudu | 999h ku |
| Yawan Electrodes | 24, sarrafa kansa |
| Yanayin Zazzabi | 0 ℃ zuwa 50 ℃, kuskuren ganowa <± 0.5 ℃ |
Bayani
Pulsed field gel electrophoresis (PFGE) yana raba kwayoyin DNA ta hanyar musanya filin lantarki tsakanin nau'ikan nau'ikan lantarki daban-daban na sararin samaniya, yana haifar da kwayoyin halittar DNA, wanda zai iya zama miliyoyin tushe nau'i-nau'i tsayin daka don sake komawa da yin ƙaura ta hanyar gelose gel pores a cikin sauri daban-daban.Yana samun babban ƙuduri a cikin wannan kewayon kuma ana amfani dashi galibi a cikin ilimin halitta;gano jinsin halittu da ƙananan ƙwayoyin cuta;bincike a cikin ilimin cututtuka na kwayoyin halitta;nazarin manyan gutsuttsura plasmid;ƙaddamar da kwayoyin cututtuka;taswirar jiki na kwayoyin halitta, nazarin RFLP, da kuma zanen yatsan DNA;shirye-shiryen binciken mutuwar kwayar halitta;nazarin kan lalacewa da gyara DNA;warewa da bincike na DNA na genomic;rabuwa na chromosomal DNA;gini, ganewa, da kuma nazarin manyan ɗakunan karatu na genomic;da bincike-bincike na transgenic.t ƙaranci kamar 0.5 ng/µL (dsDNA).
Aikace-aikace
Ya dace don ganowa da rarraba kwayoyin DNA daga 100bp zuwa 10Mb a girman, samun babban ƙuduri a cikin wannan kewayon.
Siffar
• Fasaha mai ci gaba: Haɗa fasahar CHEF da PACE pulsed-filin don cimma sakamako mafi kyau tare da madaidaiciyar hanyoyi marasa lanƙwasawa.
• Sarrafa mai zaman kanta: Siffofin 24 na'urorin lantarki na platinum masu zaman kansu (diamita 0.5mm), tare da kowane lantarki wanda za'a iya maye gurbinsa daban-daban.
• Ayyukan Lissafi ta atomatik: Haɗa maɓallan maɓalli masu yawa kamar ƙarfin ƙarfin lantarki, zafin jiki, kusurwar sauyawa, lokacin farko, lokacin ƙarshe, lokacin sauyawa na yanzu, jimlar lokacin gudu, ƙarfin lantarki, da halin yanzu don ƙididdigewa ta atomatik, yana taimaka wa masu amfani su cimma mafi kyawun yanayin gwaji.
• Algorithm Na Musamman: Yana amfani da algorithm na sarrafa bugun jini na musamman don ingantacciyar tasirin rabuwa, cikin sauƙin rarrabe tsakanin DNA na layi da madauwari, tare da haɓakar rarrabuwa na babban madauwari DNA.
• Yin aiki da kai: Yin rikodin ta atomatik da sake kunna electrophoresis idan tsarin ya katse saboda gazawar wutar lantarki.
• Mai daidaitawa: Yana ba masu amfani damar saita nasu yanayi.
• Sassautu: Tsarin zai iya zaɓar takamaiman gradients irin ƙarfin lantarki da lokutan sauyawa don takamaiman girman jeri na DNA.
• Babban allo: An sanye shi da allon LCD na 7-inch don aiki mai sauƙi, yana nuna kulawar software na musamman don amfani mai sauƙi da dacewa.
• Gane yanayin zafi: Binciken zafin jiki na dual kai tsaye yana gano zafin buffer tare da gefen kuskuren ƙasa da ± 0.5 ℃.
• Tsarin kewayawa: Ya zo tare da tsarin kewayawa na buffer wanda ke sarrafawa daidai da saka idanu da zafin jiki na buffer, yana tabbatar da yawan zafin jiki da ma'aunin ionic yayin electrophoresis.
• Babban Tsaro: Ya haɗa da murfin aminci na acrylic mai haske wanda ke yanke wuta ta atomatik lokacin da aka ɗaga shi, tare da wuce gona da iri da ayyukan kariya marasa nauyi.
• Daidaitacce Matsayi: Tankin electrophoresis da gel caster fasalin ƙafafu masu daidaitacce don daidaitawa.
• Tsarin Tsara: Ana yin tanki na electrophoresis tare da tsarin ƙirar ƙira ba tare da haɗawa ba;rakiyar lantarki sanye take da na'urorin platinum na 0.5mm, yana tabbatar da dorewa da ingantaccen sakamakon gwaji.
FAQ
Tambaya: Menene pulsed Field Gel Electrophoresis?
A: Pulsed Field Gel Electrophoresis wata dabara ce da ake amfani da ita don rarrabuwar manyan kwayoyin halittar DNA bisa girmansu.Ya ƙunshi musanya jagorancin filin lantarki a cikin matrix gel don ba da damar rabuwa da gutsuttsuran DNA waɗanda suke da girma da yawa don warware su ta hanyar al'ada agarose gel electrophoresis.
Q: Menene aikace-aikace na Pulsed Field Gel Electrophoresis?
A: pulsed Field Gel Electrophoresis ana amfani dashi sosai a cikin ilmin kwayoyin halitta da kwayoyin halitta don:
Taswirar manyan kwayoyin DNA, kamar chromosomes da plasmids.
• Ƙayyade girman kwayoyin halitta.
• Nazarin bambance-bambancen kwayoyin halitta da dangantakar juyin halitta.
• Ilimin cututtukan ƙwayoyin cuta, musamman don bin diddigin barkewar cututtuka.
• Binciken lalacewa da gyara DNA.
• Tabbatar da kasancewar takamaiman kwayoyin halitta ko jerin DNA.
Tambaya: Ta yaya Pulsed Field Gel Electrophoresis ke aiki?
A: Filayen Gel Electrophoresis na Pulsed yana aiki ta hanyar ƙaddamar da ƙwayoyin DNA zuwa filin lantarki mai bugun jini wanda ke musanya ta hanya.Wannan yana ba da damar manyan ƙwayoyin DNA su sake daidaita kansu tsakanin bugun jini, suna ba da damar motsin su ta hanyar matrix gel.Ƙananan ƙwayoyin DNA suna motsawa da sauri ta hanyar gel, yayin da mafi girma suna motsawa a hankali, suna ba da damar rabuwar su bisa girman girman.
Tambaya: Menene ka'idar bayan Pulsed Field Gel Electrophoresis?
A: Filayen Gel Electrophoresis na Pulsed yana raba kwayoyin halittar DNA bisa girmansu ta hanyar sarrafa tsawon lokaci da shugabanci na bugun wutar lantarki.Matsakaicin filin yana haifar da manyan ƙwayoyin DNA don ci gaba da sake daidaita kansu, yana haifar da ƙaura ta hanyar matrix gel da rabuwa bisa ga girman.
Q: Menene fa'idodin Pulsed Field Gel Electrophoresis?
A: Babban ƙuduri don rarraba manyan ƙwayoyin DNA har zuwa nau'i-nau'i na miliyoyin da yawa. Iyawar warwarewa da kuma bambanta gutsuttsuran DNA na nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan iri.
Tambaya: Menene kayan aiki da ake bukata don Pulsed Field Gel Electrophoresis?
A: Filayen Gel Electrophoresis yawanci yana buƙatar na'urar electrophoresis tare da na'urori na musamman don samar da filayen bugun jini.Agarose gel matrix tare da maida hankali mai dacewa da buffer.Samar da wutar lantarki mai iya haifar da bugun jini mai ƙarfi. Tsarin sanyaya don watsar da zafi da aka haifar a lokacin electrophoresis, da famfo mai kewayawa.
