Gel electrophoresis yana ɗaya daga cikin manyan hanyoyin da ake amfani da su a cikin ilimin halitta don nazarin DNA.Wannan hanya ta ƙunshi ƙaura na gutsuttsuran DNA ta hanyar gel, inda aka raba su bisa girman ko siffar su.Duk da haka, kun taɓa cin karo da wasu kurakurai a lokacin gwajin ku na electrophoresis, irin su nau'ikan da aka shafa akan gel agarose, ko babu makada akan gel?Menene zai iya zama sanadin waɗannan kurakurai?
Masana fasahar mu sun taƙaita ma'aurata na magance matsala a nan don bayanin ku.
1. Smeared makada a kan agarose gel
●An lalata DNA.Ka guji gurɓatar ƙwayar cuta.
● Mai buffer electrophoresis ba sabo bane.Bayan maimaita amfani da buffer electrophoresis, ƙarfin ionic yana raguwa, kuma ƙimar pH yana ƙaruwa, don haka ƙarfin buffer ya raunana, wanda ke rinjayar tasirin electrophoresis.Ana ba da shawarar maye gurbin ma'aunin lantarki akai-akai.
An yi amfani da yanayin electrophoresis mara kyau.Kada ka ƙyale ƙarfin lantarki ya wuce 20 V/cm, kuma kula da zafin jiki <30 ° C yayin electrophoresis.Ga giant DNA strand electrophoresis, zafin jiki ya kamata ya zama <15° C. Duba buffer electrophoresis yana da isasshen ƙarfin buffer.
● An ɗora DNA da yawa akan gel.Rage adadin DNA.
● Gishiri mai yawa a cikin DNA.Yi amfani da hazo ethanol don cire yawan gishiri a cikin ci gaba.
DNA ta gurbata da furotin.Yi amfani da cirewar phenol don cire furotin a cikin ci gaba.
● An cire DNA.Kada ku yi zafi kafin electrophoresis.Tsarma DNA a cikin buffer tare da 20 mM NaCl.
2. Anomaly DNA band hijira
● Sake fasalin shafin COS na λHind III guntu.Gasa DNA na minti 5 a ƙarƙashin 65 ° C kafin electrophoresis, sa'an nan kuma kwantar da shi a kan kankara na minti 5.
An yi amfani da yanayin electrophoresis mara kyau.Kada ka ƙyale ƙarfin lantarki ya wuce 20 V/cm, kuma kula da zafin jiki <30 ° C yayin electrophoresis.Duba buffer electrophoresis yana da isasshen ƙarfin buffer.
● An cire DNA.Kada ku yi zafi kafin electrophoresis.Tsarma DNA a cikin buffer tare da 20 mM NaCl.
3. Suma ko babu DNA makada akan gel agarose
● Babu isassun adadin ko tattarawar DNA da aka ɗora akan gel ɗin.Ƙara adadin DNA.Polyacrylamide gel electrophoresis yana da ɗan hankali fiye da agarose electrophoresis, kuma ana iya rage ɗaukar samfurin yadda ya kamata.
● DNA ɗin ya ƙasƙanta.Ka guji gurɓatar ƙwayar cuta.
An cire DNA daga gel ɗin.Electrophorese gel na ɗan lokaci, yi amfani da ƙaramin ƙarfin lantarki, ko amfani da gel mai girma.
An yi amfani da tushen hasken W mara kyau don ganin DNA mai ɗauke da ethidium bromide.Yi amfani da ɗan gajeren zangon haske (254 nm) W don ƙarin hankali.
4. Ƙwayoyin DNA sun ɓace
●Karamin girman DNA an cire shi daga gel ɗin.Electrophorese gel na ɗan lokaci, yi amfani da ƙaramin ƙarfin lantarki, ko amfani da gel mai girma.
● Yana da wahala a bambance igiyoyin DNA na kwayoyin halitta iri ɗaya.Ƙara lokacin electrophoresis, kuma duba maida hankalina gel don tabbatar da daidai adadin gel ɗin da za a yi amfani da shi.
● An cire DNA.Kada ku yi zafi kafin electrophoresis.Tsarma DNA a cikin buffer tare da 20 mM NaCl.
Matsalolin DNA suna da girma, kuma gel electrophoresis na al'ada bai dace ba.Yi nazari akan pulse gel electrophoresis.Wadanne matsaloli kuka yi da agarose gel electrophoresis?Za mu ƙara yin bincike don jagora a nan gaba.
Beijing Liuyi Biotechnology Co., Ltd (Liuyi Biotech) wani kamfani ne na musamman wanda ke mai da hankali kan samfuran da ke da alaƙa da electrophoresis a China.Labarinsa ya fara ne a shekarar 1970 lokacin da kasar Sin ba ta shiga yin gyare-gyare da bude kofa ba tukuna.Ta hanyar ci gaban shekaru, Liuyi Bitotech yana da nasa alamar, wanda aka sani da Liuyi Brand a cikin kasuwannin cikin gida don samfuran electrophoresis.
Alamar Liuyi tana da tarihin fiye da shekaru 50 a kasar Sin kuma kamfanin na iya samar da samfuran karko da inganci a duk duniya.Ta hanyar ci gaban shekaru, ya cancanci zaɓinku!
Kwayoyin electrophoresis na kwance (tankuna / ɗakuna) na Liuyi Biotech suna da inganci tare da kyan gani.Tare da daban-daban masu girma dabam na gel trays, za su iya saduwa da daban-daban na gwaji bukatun.Muna da ƙungiyar fasaha da masana'anta.Daga ƙira don ƙira, albarkatun ƙasa zuwa sassa masu mahimmanci, za mu iya sarrafa dukkan tsari.Jerin DYCP 31 na DNA electrophoresis ne, wanda samfuri neSaukewa: DYCP-31BN, Saukewa: DYCP-31CN,Saukewa: DYCP-31DN, kumaSaukewa: DYCP-31E.Bambance-bambance a tsakanin su shine girman gel da farashin.Muna ba da cikakkun nau'ikan samfurori don abokan cinikinmu.SamfurinSaukewa: DYCP-32Czai iya yin gel mafi girma 250mm * 250mm.
A halin yanzu, muna ba da shawarar samar da wutar lantarki ta electrophoresisDYY-6C,DAY-6DkumaDAY-10Cdomin mu electrophoresis Kwayoyin (tankuna / dakuna) DYCP-31 da kuma 32 jerin.
Idan kana son ƙarin bayani game da samfuran, da fatan za a ziyarci wannan gidan yanar gizon don samun ƙarin, kuma maraba da tuntuɓar mu ta imel don sanar da mu abin da kuke so, kuma ku ga ko za mu iya samar muku da mafita.
Don ƙarin bayani game da mu, da fatan za a tuntuɓe mu ta imel[email protected], [email protected].
Lokacin aikawa: Mayu-09-2022