Polyacrylamide Gel Electrophoresis

Ana amfani da polyacrylamide sau da yawa a aikace-aikacen ilimin halitta a matsayin matsakaici don electrophoresis na sunadarai da acid nucleic a wata dabara da aka sani da PAGE.Yana da wani nau'i na hanyar electrophoresis na yanki ta hanyar gel ɗin synthetics da ake kira polyacrylamide a matsayin matsakaicin tallafi.S.Raymond da L.Weintraub ne suka gina shi a shekarar 1959, sannan L.Ornstein da BJ Davis suka inganta shi kuma suka inganta shi.An yi amfani da wannan hanyar sosai bayan ƙarin bayani da gyare-gyare da su a ka'idar da fasaha na gwaji a 1964.

Kafin amfani da polyacrylamide gel electrophoresis, mutane galibi suna amfani da electrophoresis na takarda don yankin EP.Amma takarda tana aiki a matsayin matsakaici, kawai suna da aikin anti-convection, babu wani sakamako mai kyau.Yayin da polyacrylamide gel ba wai kawai yana da aikin anti-convection ba amma kuma ana iya shiga cikin aikin rabuwa da rayayye.Saboda polyacrylamide gel wani nau'i ne na tsarin net, wanda shine polymerization da haɗin haɗin haɗin gwiwa na acrylamide (Acr) da N, N-methylenebis (acrylamide).Acrylamide ana kiransa monomer, yayin da N, N-methylenebis ake kira comonomer ko crosslinker.Samar da gel wani tsari ne na polymerization na sinadaran.Ana iya sarrafa girman pore na gel, don haka za'a iya yin gel tare da digiri daban-daban na haɗin giciye.Idan girman pore yana gabatowa matsakaicin radius na kwayoyin samfurin, juriya na kwayoyin don shiga ta hanyar gel pore zai sami dangantaka ta kud da kud da girma da siffar kwayar halitta a lokacin electrophoresis.Don haka yana ba da yanayin rabuwa mai canzawa don raba waɗannan kayan tare da cajin gidan yanar gizo iri ɗaya.

Akwai manyan hanyoyi guda biyu da ake amfani da su don polyacrylamide gel electrophoresis, ɗayan diski electrophoresis, ɗayan ɗayan shine slab electrophoresis.Plate electrophoresis an ƙera shi ne musamman don raba furotin da DNA, kuma gabaɗaya, akwai nau'ikan slab electrohporesis iri biyu, waɗanda sune tankin electrophoresis na kwance da tankin electrophoresis na tsaye.Don sunadaran, mutane suna amfani da tankin electrophoresis na kwance don IFF da immunoelectrophoresis, in ba haka ba, mutane suna amfani da tankunan electrophoresis na tsaye don sunadaran.

Liuyi Biotechnology na Beijing yana da nau'ikan tankunan lantarki daban-daban don PAGE, sai dai bincike da gano samfuran furotin ta hanyar polyacrylamide gel electrophoresis, ana iya amfani da ita don auna nauyin kwayoyin halitta, samfuran tsarkakewa da shirya samfuran.

Ɗauki samfurinDYCZ-23Aa matsayin misali, wanda shine tankin electrophoresis na yau da kullun don Lab.Yi amfani da faranti biyu na gilashi don samar da dakin gel don yin gel, sannan kuma tafa farantin gilashin sosai don hana zubar gel.Kaurin gel ɗin ya dogara ne akan kauri na spacer.Yawanci, kauri shine 1.5mm don mafi kyawun sakin zafi, kumaDYCZ-23AHakanan yana ba da masu sarari kauri na 1.0mm don simintin gel 1.0mm.Sai dai tanki na electrophoresis, don gudanar da electrophoresis, ana kuma buƙatar samar da wutar lantarki.Liuyi Biotechnology na Beijing yana ba da nau'o'i iri-irisamar da wutar lantarki electrophoresis.Daga babban ƙarfin lantarki zuwa ƙananan ƙarfin lantarki, za ku zaɓi samfurin bisa ga aikace-aikacen.

Alamar Liuyi ta Beijing tana da tarihin sama da shekaru 50 a kasar Sin kuma kamfanin na iya samar da barga da kayayyaki masu inganci a duk duniya.Ta hanyar shekarun ci gaba, ya cancanci zaɓinku!

Yanzu muna neman abokan tarayya, duka OEM electrophoresis tank da masu rarraba suna maraba.

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