Mun raba ra'ayoyi da yawa a makon da ya gabata don amfani da cellulose acetate membrane electrophoresis, kuma za mu gama wannan batu a yau don tunani.
Zaɓin Matsakaicin Buffer
Matsakaicin buffer da aka yi amfani da shi a cikin cellulose acetate membrane electrophoresis gabaɗaya yana ƙasa da wanda aka yi amfani da shi a cikin electrophoresis na takarda.pH 8.6 da aka saba amfani dashiBAna zaɓi buffer mai yanke hukunci a cikin kewayon 0.05 mol/L zuwa 0.09 mol/L.Lokacin zabar maida hankali, ana yin ƙudiri na farko.Misali, idan tsayin tsiri na membrane tsakanin igiyoyi a cikin dakin electrophoresis shine 8-10cm, ana buƙatar ƙarfin lantarki na 25V kowace centimita na tsawon membrane, kuma ƙarfin halin yanzu yakamata ya zama 0.4-0.5 mA a kowace centimita na nisa membrane.Idan waɗannan dabi'u ba a samu ko sun wuce lokacin electrophoresis ba, ya kamata a ƙara yawan maida hankali ko diluted.
Ƙarƙashin ƙaƙƙarfan taro mai ƙaranci zai haifar da saurin motsi na makada da karuwa a fadin band.A gefe guda, babban taro mai girma da yawa zai rage gudun hijirar band, yana da wahala a rarrabe wasu makada na rabuwa.
Ya kamata a lura cewa a cikin cellulose acetate membrane electrophoresis, an gudanar da wani muhimmin ɓangare na halin yanzu ta hanyar samfurin, wanda ke haifar da yawan zafi.Wani lokaci, za a iya la'akari da zaɓaɓɓen maida hankali ne.Duk da haka, a ƙarƙashin yanayin ƙara yawan zafin jiki na muhalli ko lokacin amfani da mafi girman ƙarfin lantarki, ƙawancen ruwa saboda zafi zai iya ƙaruwa, yana haifar da babban ma'auni mai mahimmanci kuma har ma da haifar da membrane ya bushe.
Samfurin Girma
A cikin cellulose acetate membrane electrophoresis, adadin samfurin samfurin yana ƙayyade ta hanyoyi daban-daban, ciki har da yanayin electrophoresis, kaddarorin samfurin kanta, hanyoyin lalata, da fasaha na ganowa.A matsayin ka'ida ta gabaɗaya, mafi mahimmancin hanyar ganowa, ƙaramin ƙarar samfurin zai iya zama, wanda ke da fa'ida don rabuwa.Idan girman samfurin ya wuce kima, tsarin rabuwa na electrophoretic bazai bayyana ba, kuma tabo na iya ɗaukar lokaci.Duk da haka, lokacin da ƙididdige ƙididdige ƙididdige ƙididdiga masu ɓarna ta amfani da hanyoyin gano launi na elution, ƙimar samfurin bai kamata ya zama ƙanƙanta ba, saboda yana iya haifar da ƙananan ƙimar sha don wasu abubuwan haɗin gwiwa, yana haifar da manyan kurakurai wajen ƙididdige abun ciki.A irin waɗannan lokuta, ya kamata a ƙara ƙarar samfurin yadda ya kamata.
Yawanci, ƙarar samfurin da aka ƙara akan kowane santimita na layin aikace-aikacen samfurin ya fito daga 0.1 zuwa 5 μL, daidai da adadin samfurin 5 zuwa 1000 μg.Misali, a cikin bincike na electrophoresis na serum protein na yau da kullun, ƙimar samfurin da aka ƙara akan kowane santimita na layin aikace-aikacen gabaɗaya baya wuce 1 μL, daidai da 60 zuwa 80 μg na furotin.Koyaya, lokacin nazarin lipoproteins ko glycoproteins ta amfani da hanyar electrophoresis iri ɗaya, ana buƙatar ƙara girman samfurin daidai.
A ƙarshe, ya kamata a zaɓi ƙarar samfurin da ya fi dacewa bisa ƙayyadaddun yanayi ta hanyar gwajin gwaji na farko.
Zaɓin Maganin Taɓa
Ƙungiyoyin da aka raba a cikin cellulose acetate membrane electrophoresis yawanci suna da launi kafin ganowa.Samfurin samfuri daban-daban suna buƙatar hanyoyi daban-daban na tabo, kuma hanyoyin lalata da suka dace da cellulose acetate membrane electrophoresis maiyuwa ba za a iya amfani da su gaba ɗaya don tace takarda ba.
Akwai manyan ka'idoji guda uku don zaɓar maganin tabo doncellulose acetate membrane.Na farko,Rini mai narkewar ruwa yakamata a fifita rini masu narkewar barasa don gujewa raguwar membrane da nakasar da maganin tabo na karshen ya haifar.Bayan tabo, yana da mahimmanci don kurkura membrane da ruwa kuma rage tsawon lokacin lalata.In ba haka ba, membrane na iya zama lanƙwasa ko raguwa, wanda zai shafi ganowa na gaba.
Abu na biyu, ya fi dacewa don zaɓar dyes tare da ƙaƙƙarfan ƙaƙƙarfan ƙazanta don samfurin.A cikin cellulose acetate membrane electrophoresis na sunadaran jini, amino black 10B ana amfani dashi akai-akai saboda ƙaƙƙarfan ƙaƙƙarfan ƙaƙƙarfan ƙaƙƙarfan ƙaƙƙarfan ƙaƙƙarfan abubuwan furotin na jini da kwanciyar hankali.
Na uku, ya kamata a zaɓi rini masu inganci masu inganci.Wasu rini, duk da suna iri ɗaya, na iya ƙunsar ƙazantattun abubuwa waɗanda ke haifar da duhu musamman bayan tabo.Wannan yana iya ma ɓata makada na asali da kyau, yana sa su da wahala a rarrabe su.
A ƙarshe, zaɓin ƙaddamarwar maganin tabo yana da mahimmanci.A bisa ka'ida, yana iya zama kamar babban taro na maganin tabo zai haifar da ƙarin tabo na samfuran samfuran da kuma kyakkyawan sakamako mai kyau.Sai dai kuma ba haka lamarin yake ba.Dangantakar dauri tsakanin samfuran samfuri da rini yana da ƙayyadaddun iyaka, wanda baya ƙaruwa tare da haɓaka haɓakar ƙazamin bayani.Akasin haka, babban taro mai ɗorewa mai ɗorewa ba wai kawai yana lalata rini ba har ma yana sa ya zama da wahala a samu bayyanannen asali.Bugu da ƙari, lokacin da ƙarfin launi ya kai wani matsakaicin ƙima, rini's absorbance curve ba ya bin dangantaka ta madaidaiciya, musamman ma a cikin ma'auni na ƙididdiga.
Cikakkun bayanai don sani game da fasahar kere-kere ta Liuyi ta Beijing's cellulose acetate membraneTankin electrophoresis da aikace-aikacen sa na electrophoresis, don Allah ziyarci nan:
lGwaji don raba furotin na jini ta Cellulose Acetate Membrane
lCellulose acetate membrane Electrophoresis
lYakamata a kiyaye da yawa la'akari yayin amfani da Cellulose Acetate Membrane Electrophoresis (1)
Idan kuna da wani shirin siyan samfuranmu, da fatan a yi shakka a tuntuɓe mu.Kuna iya aiko mana da sako ta imel[email protected]ko[email protected], ko kuma a kira mu a +86 15810650221 ko ƙara Whatsapp +86 15810650221, ko Wechat: 15810650221.
Magana:Electrophoresis (bugu na biyu) na Mr. Li
Lokacin aikawa: Juni-06-2023