DYCP-40C Semi-bushe blotting tsarin ana amfani da tare da electrophoresis samar da wutar lantarki don canja wurin sunadaran a cikin polyacrylamide gels uwa da membrane kamar nitrocellulose membrane, nailan membrane da PVDF membrane.Ana yin ɓoyayyen bushe-bushe tare da na'urorin lantarki na graphite a cikin daidaitacce a kwance, yin sandwiching gel da membrane tsakanin zanen takarda mai jike da buffer wanda ke aiki azaman tafki ion.A lokacin canja wurin electrophoretic, ƙwayoyin da ba su da kyau suna yin ƙaura daga gel ɗin kuma su matsa zuwa ingantacciyar lantarki, inda aka ajiye su akan membrane.Na'urorin lantarki na farantin, wanda aka raba kawai ta hanyar gel da tace takarda, suna samar da ƙarfin filin (V / cm) a fadin gel, yana aiwatar da ingantaccen, saurin canja wuri.Canja wuri na ƙaramin DYCP - 40C electrophoresis cell shine 150 × 150 (mm), dace don canja wurin madaidaicin gels, ciki har da waɗanda daga DYCZ-24DN da DYCZ-24EN electrophoresis cell.
Bari mu ƙarin koyo game da aikin wannan na'urar trans-bushe mai bushewa.
Kayan aiki, kayan aikin DYCP-40C
Kayan wutar lantarki na Electrophoresis DYY-6C, na'urar busasshiyar trans blot DYCP-40C, maganin buffer, da kwantena don maganin buffer.da dai sauransu.
Matakan Aiki
1. Saka gel tare da faranti na gilashi a cikin maganin buffer canja wuri
2. Auna girman gel
3.Shirya takaddun takarda guda 3 bisa ga girman gel, kuma girman takarda ya kamata ya zama ɗan girma fiye da girman gel;Anan muna amfani da takarda tace Whatman;
4.Sanya takarda tace guda 3 a cikin maganin buffer sannu a hankali, kuma bari takarda tace ta nutse cikin mazugi gaba ɗaya, kuma a guji samun kumfa mai iska;
5.Shirya kuma yanke nitrocellulose membrane bisa ga girman gel da takarda tace;girman nitrocellulose membrane ya kamata ya fi girma fiye da girman gel da takarda tace;
6.Sanya membrane na nitrocellulose a cikin maganin buffer;
7.Fitar da takarda tace guda 3 sannan a sauke da karin bayani har sai wani bayani na buffer ya fado daga membrane;sa'an nan kuma sanya takarda tace a kasan DYCP-40C;
8.Ɗauki gel daga faranti na gilashi, a hankali tsaftace gel ɗin tari, kuma sanya gel a cikin maganin buffer;
9.Sanya gel a kan takarda mai tacewa, fara daga ƙarshen gel don kauce wa kumfa mai iska;
10.Yi amfani da kayan aiki mai dacewa don kawar da kumfa tsakanin gel da takarda tace.
11.Rufe murfin nitrocellulose akan gel, gefen m zuwa gel.Sannan yi amfani da kayan aiki da ya dace don kawar da kumfa na iska tsakanin membrane da gel.Saka guda 3 na takardar tacewa akan membrane.Har yanzu ana buƙatar amfani da kayan aiki da ya dace don cire kumfa na iska tsakanin takarda tace da membrane.
12.Rufe murfin, kuma saita sigogi masu gudana na electrophoresis, 80mA akai-akai;
13.Ana yin electrophoresis.Muna samun sakamakon kamar haka;
Beijing Liuyi Biotechnology Co., Ltd ya ƙware a cikin kera samfuran electrophoresis fiye da shekaru 50.Mu ne ISO9001 & ISO13485 bokan kamfanin da kuma ƙware a Manufacturing electrophoresis tankuna, da wutar lantarki, UV transilluminator, da gel takardun & tsarin bincike, a halin yanzu, muna samar da OEM sabis ga abokan ciniki, kazalika da ODM sabis.
Yanzu muna neman abokan tarayya, OEM da masu rarraba suna maraba.
Idan kuna da wani shirin siyan samfuranmu, da fatan a yi shakka a tuntuɓe mu.Kuna iya aiko mana da sako ta imel[email protected]ko[email protected], ko kuma a kira mu a +86 15810650221 ko ƙara Whatsapp +86 15810650221, ko Wechat: 15810650221.
Lokacin aikawa: Afrilu-04-2023